Research outputs per year
Research outputs per year
Description
Description and information for data files (Microtom_imaging_data.xlsx, Weights_harvest.csv, Colonisation_data.csv, Root_metabolomics.zip.) can be found in the README file. DATA & FILE OVERVIEW
File List in "Raw data single AM fungal species":
File 1 Name: Microtom_imaging_data.xlsx File 1 Description: Raw PSA/AGR imaging data and smoothed PSA (sPSA), smoothed growth rates (sPSA AGR) and relative growth rate (sPSA RGR) values for each plant and treatment.
File 2 Name: Weights_harvest.csv File 2 Description: Raw data of weights collected at harvest
File 3 Name: Colonisation_data.csv File 3 Description: Raw data collected for the calculation of AM fungal colonization percentage
File 4 Name: Root metabolomics.zip File 4 Description: Raw data collected from untargeted metabolomic analysis
DATA-SPECIFIC INFORMATION FOR File 1: Microtom_imaging_data.xlsx
Number of sheets: 5
Sheet 1: data-longi Number of columns: 9 Number of rows: 3589
Variable List Sheet: data-longi Plant: Plant ID for each experimental unit DAP: Days after planting Mycorrhiza: Type of mycorrhizal treatment including single species treatments and combination treatments Stress:Treatments that received the pathogen (Stressed) and no pathogen (Unstressed) Block: Number of each block (2 Lanes x 13 Positions) PSA: Projected shoot area (PSA), which reflects the biomass area at a specific date, quantified in kilo-pixels sPSA:smoothed PSA (sPSA) values generated by mild smoothing on the logarithmic scale sPSA AGR: Smoothed growth rates, which reflects the average growth across a defined time window, measured in kilo-pixels added from the previous day sPSA RGR: Smoothed relative growth rate
Sheet 2: data-traits Number of columns: 86 Number of rows: 157
Variable List Sheet: data-traits Plant: Plant ID for each experimental unit Mycorrhiza: ype of mycorrhizal treatment including single species treatments and combination treatments Stress: Treatments that received the pathogen (Stressed) and no pathogen (Unstressed) Block: Number of each block (2 Lanes x 13 Positions) sPSA 21: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 21 (DAP) sPSA 28: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 28 (DAP) sPSA 34: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 34 (DAP) sPSA 39: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 39 (DAP) sPSA 43: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 43 (DAP) sPSA AGR 21-28: Smoothed growth rates across the time window of 21 to 28 DAP sPSA AGR 28-34: Smoothed growth rates across the time window of 28 to 34 DAP sPSA AGR 34-39: Smoothed growth rates across the time window of 34 to 39 DAP sPSA AGR 39-43: Smoothed growth rates across the time window of 39 to 43 DAP sPSA RGR 21-28: Smoothed relative growth rates across the time window of 21 to 28 DAP sPSA RGR 28-34: Smoothed relative growth rates across the time window of 28 to 34 DAP sPSA RGR 34-39: Smoothed relative growth rates across the time window of 34 to 39 DAP sPSA RGR 39-43: Smoothed relative growth rates across the time window of 39 to 43 DAP Shoot Dry Weight: Shoot weight of the remaining shoot after sampling all other parts, measured after oven drying to remove all moisture Colonisation:Percentage of sections containing AM fungal structures (hyphae, vesicles, arbuscules) Row 20-86: Metabolomic peak identification for each detected metabolic feature of interest
Sheet 3: Pred_Interaction (predictions for the interaction model, Groups/(Mycorrhiza:Stress + Mycorrhiza Control:Stress Control)) Sheet 4: Pred_Additive (predictions for the additive model, Groups/(Mycorrhiza + Stress + Mycorrhiza Control + StressControl)) Sheet 5: Preds_Chosen (predictions for the chosen model, (Mycorrhiza:Stress, Mycorrhiza, Stress, Mycorrhiza Control: Stress Control, Mycorrhiza Control, Stress Control, Overall mean)
Number of columns in sheet 3, 4 & 5: 248 Number of rows in sheet 3, 4 & 5: 15
Variable List Sheet: Pred_Interaction, Pred_Additive & Preds_Chosen
Mycorrhiza: Type of mycorrhizal treatment including single species treatments and combination treatments
Stress: Treatments that received the pathogen (Stressed) and no pathogen (Unstressed)
sPSA 21.pred: Predictions obtained from models at sPSA 21
sPSA 21.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 21
sPSA 21.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 21
sPSA 28.pred: Predictions obtained from models at sPSA 28
sPSA 28.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 28
sPSA 28.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 28
sPSA 34.pred: Predictions obtained from models at sPSA 34
sPSA 34.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 34
sPSA 34.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 34
sPSA 39.pred: Predictions obtained from models at sPSA 39
sPSA 39.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 39
sPSA 39.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 39
sPSA 43.pred: Predictions obtained from models at sPSA 43
sPSA 43.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 43
sPSA 43.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 43
sPSA AGR 21-28.pred: Predictions obtained from models at sPSA AGR 21-28
sPSA AGR 21-28.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 21-28
sPSA AGR 28-34.pred: Predictions obtained from models at sPSA AGR 28-34
sPSA AGR 28-34.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA AGR 28-34
sPSA AGR 28-34.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 28-34
sPSA AGR 34-39.pred: Predictions obtained from models at sPSA AGR 34-39
sPSA AGR 34-39.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA AGR 34-39
sPSA AGR 34-39.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 34-39
sPSA AGR 39-43.pred: Predictions obtained from models at sPSA AGR 39-43
sPSA AGR 39-43.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA AGR 39-43
sPSA AGR 39-43.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 39-43
sPSA RGR 21-28.pred: Predictions obtained from models at sPSA RGR 21-28
sPSA RGR 21-28.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 21-28
sPSA RGR 21-28.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 21-28
sPSA RGR 28-34.pred: Predictions obtained from models at sPSA RGR 28-34
sPSA RGR 28-34.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 28-34
sPSA RGR 28-34.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 28-34
sPSA RGR 34-39.pred: Predictions obtained from models at sPSA RGR 34-39
sPSA RGR 34-39.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 34-39
sPSA RGR 34-39.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 34-39
sPSA RGR 39-43.pred: Predictions obtained from models at sPSA RGR 39-43
sPSA RGR 39-43.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 39-43
sPSA RGR 39-43.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 39-43
Shoot Dry Weight.pred: Predictions obtained from models for shoot dry weight
Shoot Dry Weight.lowlim.lsd: Lower limit value for the LSD (5%) for shoot dry weight
Shoot Dry Weight.uplim.lsd: Upper limit value for the LSD (5%) for shoot dry weight
Colonisation.pred: Predictions obtained from models for percentage of sections colonised by AM fungi
Colonisation.lowlim.lsd: Lower limit value for the LSD (5%) for percentage of sections colonised by AM fungi
Colonisation.uplim.lsd: Upper limit value for the LSD (5%) for percentage of sections colonised by AM fungi
Row 48-248: Predictions obtained from models, lower and upper limit for the LSD(5%) for each detected metabolic feature
DATA-SPECIFIC INFORMATION FOR File 2: Weights_harvest.csv
Number of columns: 14 Number of rows: 157
Variable List: Barcode: Plant ID for each experimental unit Print Info: Labels on pots in the greenhouse with information indicating their lane and position for clear identification Sample Nr: Number on pots in the greenhouse with information for clear identification Shoot weight in g: Fresh shoot weight in grams Root qPCR: Weight in grams of root material collected for qPCR analysis Root (casette): Weight in grams of root material collected for root staining and percentage colonization analysis Metabolomics Apical meristem: Weight in grams of material collected from the apical meristem for metabolomic analysis Metabolomics Shoot: Weight in grams of material collected from the shoots for metabolomic analysis Metabolomics Root: Weight in grams of material collected from the roots for metabolomic analysis. SDW (shoot dry weight): Shoot weight of the remaining shoot after sampling all other parts, measured after oven drying to remove all moisture notes: Additional notes taken at harvest, including details on samples used for pathogen re-isolation or fruit establishment on pl This file contains more than 10 kB. To see the remaining content please download the original file.
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Readme.txt (11.8 kB)
File List in "Raw data single AM fungal species":
File 1 Name: Microtom_imaging_data.xlsx File 1 Description: Raw PSA/AGR imaging data and smoothed PSA (sPSA), smoothed growth rates (sPSA AGR) and relative growth rate (sPSA RGR) values for each plant and treatment.
File 2 Name: Weights_harvest.csv File 2 Description: Raw data of weights collected at harvest
File 3 Name: Colonisation_data.csv File 3 Description: Raw data collected for the calculation of AM fungal colonization percentage
File 4 Name: Root metabolomics.zip File 4 Description: Raw data collected from untargeted metabolomic analysis
DATA-SPECIFIC INFORMATION FOR File 1: Microtom_imaging_data.xlsx
Number of sheets: 5
Sheet 1: data-longi Number of columns: 9 Number of rows: 3589
Variable List Sheet: data-longi Plant: Plant ID for each experimental unit DAP: Days after planting Mycorrhiza: Type of mycorrhizal treatment including single species treatments and combination treatments Stress:Treatments that received the pathogen (Stressed) and no pathogen (Unstressed) Block: Number of each block (2 Lanes x 13 Positions) PSA: Projected shoot area (PSA), which reflects the biomass area at a specific date, quantified in kilo-pixels sPSA:smoothed PSA (sPSA) values generated by mild smoothing on the logarithmic scale sPSA AGR: Smoothed growth rates, which reflects the average growth across a defined time window, measured in kilo-pixels added from the previous day sPSA RGR: Smoothed relative growth rate
Sheet 2: data-traits Number of columns: 86 Number of rows: 157
Variable List Sheet: data-traits Plant: Plant ID for each experimental unit Mycorrhiza: ype of mycorrhizal treatment including single species treatments and combination treatments Stress: Treatments that received the pathogen (Stressed) and no pathogen (Unstressed) Block: Number of each block (2 Lanes x 13 Positions) sPSA 21: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 21 (DAP) sPSA 28: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 28 (DAP) sPSA 34: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 34 (DAP) sPSA 39: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 39 (DAP) sPSA 43: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 43 (DAP) sPSA AGR 21-28: Smoothed growth rates across the time window of 21 to 28 DAP sPSA AGR 28-34: Smoothed growth rates across the time window of 28 to 34 DAP sPSA AGR 34-39: Smoothed growth rates across the time window of 34 to 39 DAP sPSA AGR 39-43: Smoothed growth rates across the time window of 39 to 43 DAP sPSA RGR 21-28: Smoothed relative growth rates across the time window of 21 to 28 DAP sPSA RGR 28-34: Smoothed relative growth rates across the time window of 28 to 34 DAP sPSA RGR 34-39: Smoothed relative growth rates across the time window of 34 to 39 DAP sPSA RGR 39-43: Smoothed relative growth rates across the time window of 39 to 43 DAP Shoot Dry Weight: Shoot weight of the remaining shoot after sampling all other parts, measured after oven drying to remove all moisture Colonisation:Percentage of sections containing AM fungal structures (hyphae, vesicles, arbuscules) Row 20-86: Metabolomic peak identification for each detected metabolic feature of interest
Sheet 3: Pred_Interaction (predictions for the interaction model, Groups/(Mycorrhiza:Stress + Mycorrhiza Control:Stress Control)) Sheet 4: Pred_Additive (predictions for the additive model, Groups/(Mycorrhiza + Stress + Mycorrhiza Control + StressControl)) Sheet 5: Preds_Chosen (predictions for the chosen model, (Mycorrhiza:Stress, Mycorrhiza, Stress, Mycorrhiza Control: Stress Control, Mycorrhiza Control, Stress Control, Overall mean)
Number of columns in sheet 3, 4 & 5: 248 Number of rows in sheet 3, 4 & 5: 15
Variable List Sheet: Pred_Interaction, Pred_Additive & Preds_Chosen
Mycorrhiza: Type of mycorrhizal treatment including single species treatments and combination treatments
Stress: Treatments that received the pathogen (Stressed) and no pathogen (Unstressed)
sPSA 21.pred: Predictions obtained from models at sPSA 21
sPSA 21.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 21
sPSA 21.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 21
sPSA 28.pred: Predictions obtained from models at sPSA 28
sPSA 28.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 28
sPSA 28.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 28
sPSA 34.pred: Predictions obtained from models at sPSA 34
sPSA 34.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 34
sPSA 34.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 34
sPSA 39.pred: Predictions obtained from models at sPSA 39
sPSA 39.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 39
sPSA 39.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 39
sPSA 43.pred: Predictions obtained from models at sPSA 43
sPSA 43.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 43
sPSA 43.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 43
sPSA AGR 21-28.pred: Predictions obtained from models at sPSA AGR 21-28
sPSA AGR 21-28.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 21-28
sPSA AGR 28-34.pred: Predictions obtained from models at sPSA AGR 28-34
sPSA AGR 28-34.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA AGR 28-34
sPSA AGR 28-34.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 28-34
sPSA AGR 34-39.pred: Predictions obtained from models at sPSA AGR 34-39
sPSA AGR 34-39.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA AGR 34-39
sPSA AGR 34-39.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 34-39
sPSA AGR 39-43.pred: Predictions obtained from models at sPSA AGR 39-43
sPSA AGR 39-43.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA AGR 39-43
sPSA AGR 39-43.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 39-43
sPSA RGR 21-28.pred: Predictions obtained from models at sPSA RGR 21-28
sPSA RGR 21-28.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 21-28
sPSA RGR 21-28.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 21-28
sPSA RGR 28-34.pred: Predictions obtained from models at sPSA RGR 28-34
sPSA RGR 28-34.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 28-34
sPSA RGR 28-34.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 28-34
sPSA RGR 34-39.pred: Predictions obtained from models at sPSA RGR 34-39
sPSA RGR 34-39.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 34-39
sPSA RGR 34-39.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 34-39
sPSA RGR 39-43.pred: Predictions obtained from models at sPSA RGR 39-43
sPSA RGR 39-43.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 39-43
sPSA RGR 39-43.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 39-43
Shoot Dry Weight.pred: Predictions obtained from models for shoot dry weight
Shoot Dry Weight.lowlim.lsd: Lower limit value for the LSD (5%) for shoot dry weight
Shoot Dry Weight.uplim.lsd: Upper limit value for the LSD (5%) for shoot dry weight
Colonisation.pred: Predictions obtained from models for percentage of sections colonised by AM fungi
Colonisation.lowlim.lsd: Lower limit value for the LSD (5%) for percentage of sections colonised by AM fungi
Colonisation.uplim.lsd: Upper limit value for the LSD (5%) for percentage of sections colonised by AM fungi
Row 48-248: Predictions obtained from models, lower and upper limit for the LSD(5%) for each detected metabolic feature
DATA-SPECIFIC INFORMATION FOR File 2: Weights_harvest.csv
Number of columns: 14 Number of rows: 157
Variable List: Barcode: Plant ID for each experimental unit Print Info: Labels on pots in the greenhouse with information indicating their lane and position for clear identification Sample Nr: Number on pots in the greenhouse with information for clear identification Shoot weight in g: Fresh shoot weight in grams Root qPCR: Weight in grams of root material collected for qPCR analysis Root (casette): Weight in grams of root material collected for root staining and percentage colonization analysis Metabolomics Apical meristem: Weight in grams of material collected from the apical meristem for metabolomic analysis Metabolomics Shoot: Weight in grams of material collected from the shoots for metabolomic analysis Metabolomics Root: Weight in grams of material collected from the roots for metabolomic analysis. SDW (shoot dry weight): Shoot weight of the remaining shoot after sampling all other parts, measured after oven drying to remove all moisture notes: Additional notes taken at harvest, including details on samples used for pathogen re-isolation or fruit establishment on pl This file contains more than 10 kB. To see the remaining content please download the original file.
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Readme.txt (11.8 kB)
| Date made available | 14 Jan 2025 |
|---|---|
| Publisher | Western Sydney University |
Research output
- 1 Article
-
Plant phenotyping and root-associated metabolomics reveal insights into pathogen protection by diverse arbuscular mycorrhizal fungi
Weinberger, N. V., Cibils-Stewart, X., Brien, C., Jewell, N., Berger, B., Cavagnaro, T. R., Salomon, M. J., Mikhael, M., Plett, J. M., Plett, K. L. & Powell, J. R., 2025, (E-pub ahead of print (In Press)) In: Plants People Planet. 15 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile