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Raw data single AM fungal species

  • Natascha Weinberger (Creator)

    Dataset

    Description

    Description and information for data files (Microtom_imaging_data.xlsx, Weights_harvest.csv, Colonisation_data.csv, Root_metabolomics.zip.) can be found in the README file. DATA & FILE OVERVIEW

    File List in "Raw data single AM fungal species":

    File 1 Name: Microtom_imaging_data.xlsx File 1 Description: Raw PSA/AGR imaging data and smoothed PSA (sPSA), smoothed growth rates (sPSA AGR) and relative growth rate (sPSA RGR) values for each plant and treatment.

    File 2 Name: Weights_harvest.csv File 2 Description: Raw data of weights collected at harvest

    File 3 Name: Colonisation_data.csv File 3 Description: Raw data collected for the calculation of AM fungal colonization percentage

    File 4 Name: Root metabolomics.zip File 4 Description: Raw data collected from untargeted metabolomic analysis

    DATA-SPECIFIC INFORMATION FOR File 1: Microtom_imaging_data.xlsx

    Number of sheets: 5

    Sheet 1: data-longi Number of columns: 9 Number of rows: 3589

    Variable List Sheet: data-longi Plant: Plant ID for each experimental unit DAP: Days after planting Mycorrhiza: Type of mycorrhizal treatment including single species treatments and combination treatments Stress:Treatments that received the pathogen (Stressed) and no pathogen (Unstressed) Block: Number of each block (2 Lanes x 13 Positions) PSA: Projected shoot area (PSA), which reflects the biomass area at a specific date, quantified in kilo-pixels sPSA:smoothed PSA (sPSA) values generated by mild smoothing on the logarithmic scale sPSA AGR: Smoothed growth rates, which reflects the average growth across a defined time window, measured in kilo-pixels added from the previous day sPSA RGR: Smoothed relative growth rate

    Sheet 2: data-traits Number of columns: 86 Number of rows: 157

    Variable List Sheet: data-traits Plant: Plant ID for each experimental unit Mycorrhiza: ype of mycorrhizal treatment including single species treatments and combination treatments Stress: Treatments that received the pathogen (Stressed) and no pathogen (Unstressed) Block: Number of each block (2 Lanes x 13 Positions) sPSA 21: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 21 (DAP) sPSA 28: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 28 (DAP) sPSA 34: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 34 (DAP) sPSA 39: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 39 (DAP) sPSA 43: smoothed PSA values generated by mild smoothing on the logarithmic scale on day 43 (DAP) sPSA AGR 21-28: Smoothed growth rates across the time window of 21 to 28 DAP sPSA AGR 28-34: Smoothed growth rates across the time window of 28 to 34 DAP sPSA AGR 34-39: Smoothed growth rates across the time window of 34 to 39 DAP sPSA AGR 39-43: Smoothed growth rates across the time window of 39 to 43 DAP sPSA RGR 21-28: Smoothed relative growth rates across the time window of 21 to 28 DAP sPSA RGR 28-34: Smoothed relative growth rates across the time window of 28 to 34 DAP sPSA RGR 34-39: Smoothed relative growth rates across the time window of 34 to 39 DAP sPSA RGR 39-43: Smoothed relative growth rates across the time window of 39 to 43 DAP Shoot Dry Weight: Shoot weight of the remaining shoot after sampling all other parts, measured after oven drying to remove all moisture Colonisation:Percentage of sections containing AM fungal structures (hyphae, vesicles, arbuscules) Row 20-86: Metabolomic peak identification for each detected metabolic feature of interest

    Sheet 3: Pred_Interaction (predictions for the interaction model, Groups/(Mycorrhiza:Stress + Mycorrhiza Control:Stress Control)) Sheet 4: Pred_Additive (predictions for the additive model, Groups/(Mycorrhiza + Stress + Mycorrhiza Control + StressControl)) Sheet 5: Preds_Chosen (predictions for the chosen model, (Mycorrhiza:Stress, Mycorrhiza, Stress, Mycorrhiza Control: Stress Control, Mycorrhiza Control, Stress Control, Overall mean)

    Number of columns in sheet 3, 4 & 5: 248 Number of rows in sheet 3, 4 & 5: 15

    Variable List Sheet: Pred_Interaction, Pred_Additive & Preds_Chosen

    Mycorrhiza: Type of mycorrhizal treatment including single species treatments and combination treatments
    Stress: Treatments that received the pathogen (Stressed) and no pathogen (Unstressed)
    sPSA 21.pred: Predictions obtained from models at sPSA 21
    sPSA 21.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 21
    sPSA 21.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 21
    sPSA 28.pred: Predictions obtained from models at sPSA 28
    sPSA 28.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 28
    sPSA 28.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 28
    sPSA 34.pred: Predictions obtained from models at sPSA 34
    sPSA 34.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 34
    sPSA 34.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 34
    sPSA 39.pred: Predictions obtained from models at sPSA 39
    sPSA 39.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 39
    sPSA 39.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 39
    sPSA 43.pred: Predictions obtained from models at sPSA 43
    sPSA 43.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA 43
    sPSA 43.uplim.lsd: Upper limit value for the LSD (5%) at sPSA 43
    sPSA AGR 21-28.pred: Predictions obtained from models at sPSA AGR 21-28
    sPSA AGR 21-28.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 21-28
    sPSA AGR 28-34.pred: Predictions obtained from models at sPSA AGR 28-34
    sPSA AGR 28-34.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA AGR 28-34
    sPSA AGR 28-34.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 28-34
    sPSA AGR 34-39.pred: Predictions obtained from models at sPSA AGR 34-39
    sPSA AGR 34-39.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA AGR 34-39
    sPSA AGR 34-39.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 34-39
    sPSA AGR 39-43.pred: Predictions obtained from models at sPSA AGR 39-43
    sPSA AGR 39-43.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA AGR 39-43
    sPSA AGR 39-43.uplim.lsd: Upper limit value for the LSD (5%) at sPSA AGR 39-43
    sPSA RGR 21-28.pred: Predictions obtained from models at sPSA RGR 21-28
    sPSA RGR 21-28.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 21-28
    sPSA RGR 21-28.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 21-28
    sPSA RGR 28-34.pred: Predictions obtained from models at sPSA RGR 28-34
    sPSA RGR 28-34.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 28-34
    sPSA RGR 28-34.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 28-34
    sPSA RGR 34-39.pred: Predictions obtained from models at sPSA RGR 34-39
    sPSA RGR 34-39.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 34-39
    sPSA RGR 34-39.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 34-39
    sPSA RGR 39-43.pred: Predictions obtained from models at sPSA RGR 39-43
    sPSA RGR 39-43.lowlim.lsd: Lower limit value for the LSD (5%) at sPSA RGR 39-43
    sPSA RGR 39-43.uplim.lsd: Upper limit value for the LSD (5%) at sPSA RGR 39-43
    Shoot Dry Weight.pred: Predictions obtained from models for shoot dry weight
    Shoot Dry Weight.lowlim.lsd: Lower limit value for the LSD (5%) for shoot dry weight
    Shoot Dry Weight.uplim.lsd: Upper limit value for the LSD (5%) for shoot dry weight
    Colonisation.pred: Predictions obtained from models for percentage of sections colonised by AM fungi
    Colonisation.lowlim.lsd: Lower limit value for the LSD (5%) for percentage of sections colonised by AM fungi
    Colonisation.uplim.lsd: Upper limit value for the LSD (5%) for percentage of sections colonised by AM fungi
    Row 48-248: Predictions obtained from models, lower and upper limit for the LSD(5%) for each detected metabolic feature
    DATA-SPECIFIC INFORMATION FOR File 2: Weights_harvest.csv

    Number of columns: 14 Number of rows: 157

    Variable List: Barcode: Plant ID for each experimental unit Print Info: Labels on pots in the greenhouse with information indicating their lane and position for clear identification Sample Nr: Number on pots in the greenhouse with information for clear identification Shoot weight in g: Fresh shoot weight in grams Root qPCR: Weight in grams of root material collected for qPCR analysis Root (casette): Weight in grams of root material collected for root staining and percentage colonization analysis Metabolomics Apical meristem: Weight in grams of material collected from the apical meristem for metabolomic analysis Metabolomics Shoot: Weight in grams of material collected from the shoots for metabolomic analysis Metabolomics Root: Weight in grams of material collected from the roots for metabolomic analysis. SDW (shoot dry weight): Shoot weight of the remaining shoot after sampling all other parts, measured after oven drying to remove all moisture notes: Additional notes taken at harvest, including details on samples used for pathogen re-isolation or fruit establishment on pl This file contains more than 10 kB. To see the remaining content please download the original file.

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    Readme.txt (11.8 kB)
    Date made available14 Jan 2025
    PublisherWestern Sydney University

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