Roland Gamsjaeger

2024

A biochemical and biophysical analysis of the interaction of nsp9 with nsp12 from SARS-CoV-2 : implications for future drug discovery efforts

Baker, D. L., Wang, B., Wilkinson-White, L. E., El-Kamand, S., Allport, T. A., Ataide, S. F., Kwan, A. H., Artsimovitch, I., Cubeddu, L. & Gamsjaeger, R., 2024, In: Proteins : Structure, Function, and Bioinformatics. 92, 11, p. 1308-1317 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

A structural analysis of the nsp9 protein from the coronavirus MERS CoV reveals a conserved RNA binding interface

Mani, G., El-Kamand, S., Wang, B., Baker, D. L., Ataide, S. F., Artsimovitch, I., Cubeddu, L. & Gamsjaeger, R., Mar 2024, In: Proteins. p. 418-426 9 p.

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Sequence- and structure-dependent cytotoxicity of phosphorothioate and 2¢-O-methyl modified single-stranded oligonucleotides

Croft, L. V., Fisher, M., Barbhuiya, T. K., El-Kamand, S., Beard, S., Rajapakse, A., Gamsjaeger, R., Cubeddu, L., Bolderson, E., O'Byrne, K., Richard, D. & Gandhi, N. S., 1 Jun 2024, In: Nucleic Acid Therapeutics. 34, 3, p. 143-155 13 p.

Research output: Contribution to journal › Article › peer-review

2023

The molecular details of a novel phosphorylation-dependent interaction between MRN and the SOSS complex

El-Kamand, S., Adams, M. N., Matthews, J. M., Du Plessis, M.-D., Crossett, B., Connolly, A., Breen, N., Dudley, A., Richard, D. J., Gamsjaeger, R. & Cubeddu, L., Oct 2023, In: Protein Science. 32, 10, 12 p., e4782.

Research output: Contribution to journal › Article › peer-review

Open Access

2 Citations (Scopus)

2022

A distinct ssDNA/RNA binding interface in the Nsp9 protein from SARS-CoV-2

El-Kamand, S., Du Plessis, M.-D., Breen, N., Johnson, L., Beard, S., Kwan, A. H., Richard, D. J., Cubeddu, L. & Gamsjaeger, R., 2022, In: Proteins. 90, 1, p. 176-185 10 p.

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Essential role of histidine for rapid copper(II)-mediated disassembly of neurokinin B amyloid

Jayawardena, B. M., Peacey, L., Gamsjaeger, R. & Jones, C. E., 2022, In: Biomolecules. 12, 11, 11 p., 1585.

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

2021

Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A)

El-Kamand, S., Du Plessis, M.-D., Lawson, T., Cubeddu, L. & Gamsjaeger, R., 2021, Single Stranded DNA Binding Proteins. Oliveira, M. T. (ed.). U.S.: Humana Press, p. 229-240 12 p.

Research output: Chapter in Book / Conference Paper › Chapter

hSSB2 (NABP1) is required for the recruitment of RPA during the cellular response to DNA UV damage

Boucher, D., Kariawasam, R., Burgess, J., Gimenez, A., Ocampo, T. E., Ferguson, B., Naqi, A., Walker, G. J., Bolderson, E., Gamsjaeger, R., O’Byrne, K. J., Cubeddu, L., Khanna, K. K. & Richard, D. J., 2021, In: Scientific Reports. 11, 1, 12 p., 20256.

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

2019

A structural perspective on the regulation of human single-stranded DNA binding protein 1 (hSSB1, OBFC2B) function in DNA repair

Lawson, T., El-Kamand, S., Kariawasam, R., Richard, D. J., Cubeddu, L. & Gamsjaeger, R., 2019, In: Computational and Structural Biotechnology Journal. 17, p. 441-446 6 p.

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Human single-stranded DNA binding protein 1 (hSSB1, OBFC2B) : a critical component of the DNA damage response

Croft, L. V., Bolderson, E., Adams, M. N., El-Kamand, S., Kariawasam, R., Cubeddu, L., Gamsjaeger, R. & Richard, D. J., 2019, In: Seminars in Cell and Developmental Biology. 86, p. 121-128 8 p.

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

The structural details of the interaction of single-stranded DNA binding protein hSSB2 (NABP1/OBFC2A) with UV-damaged DNA

Lawson, T., El-Kamand, S., Boucher, D., Duong, D. C., Kariawasam, R., Bonvin, A. M. J. J., Richard, D. J., Gamsjaeger, R. & Cubeddu, L., 2019, In: Proteins. 88, 2, p. 319-326 8 p.

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

2018

Backbone 1H, 13C and 15N resonance assignments of the OB domain of the single stranded DNA-binding protein hSSB2 (NABP1/OBFC2A) and chemical shift mapping of the DNA-binding interface

Kariawasam, R., Knight, M., Gamsjaeger, R. & Cubeddu, L., 2018, In: Biomolecular NMR Assignments. 12, 1, p. 107-111 5 p.

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

The BRD3 ET domain recognizes a short peptide motif through a mechanism that is conserved across chromatin remodelers and transcriptional regulators

Wai, D. C., Szyszka, T. N., Campbell, A. E., Kwong, C., Wilkinson-White, L. E., Silva, A. P. G., Low, J. K. K., Kwan, A. H., Gamsjaeger, R., Chalmers, J. N., Patrick, W. N., Lu, B., Vakoc, C. R., Blobel, G. A. & Mackay, J. P., 2018, In: Journal of Biological Chemistry. 293, 19, p. 7160-7175 16 p.

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

2017

A data-driven structural model of hSSB1 (NABP2/OBFC2B) self-oligomerization

Touma, C., Adams, M. N., Ashton, N. W., Mizzi, M., El-Kamand, S., Richard, D. J., Cubeddu, L. & Gamsjaeger, R., 2017, In: Nucleic Acids Research. 45, 14, p. 8609-8620 12 p.

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

High-affinity RNA binding by a hyperthermophilic single-stranded DNA-binding protein

Morten, M. J., Gamsjaeger, R., Cubeddu, L., Kariawasam, R., Peregrina, J., Penedo, J. C. & White, M. F., 2017, In: Extremophiles. 21, 2, p. 369-379 11 p.

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

hSSB1 phosphorylation is dynamically regulated by DNA-PK and PPP-family protein phosphatases

Ashton, N. W., Paquet, N., Shirran, S. L., Bolderson, E., Kariawasam, R., Touma, C., Fallahbaghery, A., Gamsjaeger, R., Cubeddu, L., Botting, C., Pollock, P. M., O’Byrne, K. J. & Richard, D. J., 2017, In: DNA Repair. 54, p. 30-39 10 p.

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

2016

A structural analysis of DNA binding by hSSB1 (NABP2/OBFC2B) in solution

Touma, C., Kariawasam, R., Gimenez, A. X., Bernado, R. E., Ashton, N. W., Adams, M. N., Paquet, N., Cubeddu, L., Gamsjaeger, R. & [and three others], 2016, In: Nucleic Acids Research. 44, 16, p. 7963-7973 11 p.

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Backbone 1H, 13C and 15N resonance assignments of the OB domain of the single stranded DNA-binding protein hSSB1 (NABP2/OBFC2B) and chemical shift mapping of the DNA-binding interface

Kariawasam, R., Touma, C., Cubeddu, L. & Gamsjaeger, R., 2016, In: Biomolecular NMR Assignments. 10, 2, p. 297-300 4 p.

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress

Paquet, N., Adams, M. N., Ashton, N. W., Touma, C., Gamsjaeger, R., Cubeddu, L., Leong, V., Beard, S., Bolderson, E., Botting, C. H., O’Byrne, K. J. & Richard, D. J., 2016, In: Scientific Reports. 6, 13 p., 27446.

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

The binding of syndapin SH3 domain to dynamin proline-rich domain involves short and long distance elements

Luo, L., Xue, J., Kwan, A., Gamsjaeger, R., Wielens, J., Kleist, L. V., Cubeddu, L., Guo, Z., Stow, J. L., Parker, M. W., Mackay, J. P. & Robinson, P. J., 2016, In: Journal of Biological Chemistry. 291, 18, p. 9411-9424 14 p.

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

2015

Biophysical characterisation and quantification of nucleic acid-protein interactions : EMSA, MST and SPR

Flores, J. K., Kariawasam, R., Gimenez, A. X., Helder, S., Cubeddu, L., Gamsjaeger, R. & Ataide, S. F., 2015, In: Current Protein and Peptide Science. 16, 8, p. 727-734 8 p.

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

hSSB1 (NABP2/ OBFC2B) is required for the repair of 8-oxo-guanine by the hOGG1-mediated base excision repair pathway

Paquet, N., Adams, M. N., Leong, V., Ashton, N. W., Touma, C., Gamsjaeger, R., Cubeddu, L., Beard, S., Burgess, J. T., Bolderson, E., O’Byrne, K. J. & Richard, D. J., 2015, In: Nucleic Acids Research. 43, 18, p. 8817-8829 13 p.

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Site directed nitroxide spin labeling of oligonucleotides for NMR and EPR studies

Shepherd, N. E., Gamsjaeger, R., Vandevenne, M., Cubeddu, L. & Mackay, J. P., 2015, In: Tetrahedron. 71, 5, p. 813-819 7 p.

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

The structural basis of DNA binding by the single stranded DNA binding protein from Sulfolobus solfataricus

Gamsjaeger, R., Kariawasam, R., Gimenez, A. X., Touma, C., McIlwain, E., Bernardo, R. E., Shepherd, N. E., Ataide, S. F., Dong, Q., Richard, D. J., White, M. F. & Cubeddu, L., 2015, In: Biochemical Journal. 465, 2, p. 337-346 19 p.

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

2014

Backbone and side-chain 1H, 13C and 15N resonance assignments of the OB domain of the single stranded DNA binding protein from Sulfolobus solfataricus and chemical shift mapping of the DNA-binding interface

Gamsjaeger, R., Kariawasam, R., Touma, C., Kwan, A. H., White, M. F. & Cubeddu, L., 2014, In: Biomolecular NMR Assignments. 8, p. 243-246 4 p., 2.

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

2013

A structural analysis of DNA binding by myelin transcription factor 1 double zinc fingers

Gamsjaeger, R., O'Connell, M. R., Cubeddu, L., Shepherd, N. E., Lowry, J. A., Kwan, A. H., Vandevenne, M., Swanton, M. K., Matthews, J. M. & Mackay, J. P., 2013, In: Journal of Biological Chemistry. 288, 49, p. 35180-35191 12 p.

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Semi-quantitative and quantitative analysis of protein-DNA interactions using steady-state measurements in surface plasmon resonance competition experiments

Gamsjaeger, R., Kariawasam, R., Bang, L. H., Touma, C., Nguyen, C. D., Matthews, J. M., Cubeddu, L. & Mackay, J. P., 2013, In: Analytical Biochemistry. 440, 2, p. 178-185 8 p.

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

2012

Modular assembly of RanBP2-Type zinc finger domains to target single-stranded RNA

O'Connell, M. R., Vandevenne, M., Nguyen, C. D., Matthews, J. M., Gamsjaeger, R., Segal, D. J. & MacKay, J. P., 2012, In: Angewandte Chemie (International Edition). 51, 22, p. 5371-5375 5 p.

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Solution structure of a tethered Lmo2LIM2/Ldb1LID complex

Dastmalchi, S., Wilkinson-White, L., Kwan, A. H., Gamsjaeger, R., Mackay, J. P. & Matthews, J. M., 2012, In: Protein Science. 21, 11, p. 1768-1774 7 p.

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

2011

A dynamic pharmacophore drives the interaction between psalmotoxin-1 and the putative drug target acid-sensing ion channel 1a

Saez, N. J., Mobli, M., Bieri, M., Chassagnon, I. R., Malde, A. K., Gamsjaeger, R., Mark, A. E., Gooley, P. R., Rash, L. D. & King, G. F., 2011, In: Molecular Pharmacology. 80, 5, p. 796-808 13 p.

Research output: Contribution to journal › Article › peer-review

76 Citations (Scopus)

Bromodomain protein Brd3 associates with acetylated GATA1 to promote its chromatin occupancy at erythroid target genes

Lamonica, J. M., Deng, W., Kadauke, S., Campbell, A. E., Gamsjaeger, R., Wang, H., Cheng, Y., Billin, A. N., Hardison, R. C., Mackay, J. P. & Blobel, G. A., 2011, In: Proceedings of the National Academy of Sciences of the United States of America. 108, 22, p. E159-E168 10 p.

Research output: Contribution to journal › Article › peer-review

189 Citations (Scopus)

Structural basis and specificity of acetylated transcription factor GATA1 recognition by BET family bromodomain protein Brd3

Gamsjaeger, R., Webb, S. R., Lamonica, J. M., Billin, A., Blobel, G. A. & Mackay, J. P., 2011, In: Molecular and Cellular Biology. 31, 13, p. 2632-2640 9 p.

Research output: Contribution to journal › Article › peer-review

102 Citations (Scopus)

Structural basis of simultaneous recruitment of the transcriptional regulators LMO2 and FOG1/ZFPM1 by the transcription factor GATA1

Wilkinson-White, L., Gamsjaeger, R., Dastmalchi, S., Wienert, B., Stokes, P. H., Crossley, M., Mackay, J. P. & Matthews, J. M., 2011, In: Proceedings of the National Academy of Sciences of the United States of America. 108, 35, p. 14443-14448 6 p.

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

The modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis

Li, N., Yun, P., Jeffries, C. M., Langley, D., Gamsjaeger, R., Church, W. B., Hunter, N. & Collyer, C. A., 2011, In: Molecular Microbiology. 81, 5, p. 1358-1373 16 p.

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

2009

Structural analysis of MED-1 reveals unexpected diversity in the mechanism of DNA recognition by GATA-type zinc finger domains

Lowry, J. A., Gamsjaeger, R., Thong, S. Y., Hung, W., Kwan, A. H., Broitman-Maduro, G., Matthews, J. M., Maduro, M. & Mackay, J. P., 2009, In: Journal of Biological Chemistry. 284, 9, p. 5827-5835 9 p.

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

The structural analysis of protein-protein interactions by NMR spectroscopy

O'Connell, M. R., Gamsjaeger, R. & Mackay, J. P., 2009, In: Proteomics. 9, 23, p. 5224-5232 9 p.

Research output: Contribution to journal › Article › peer-review

76 Citations (Scopus)

The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences

Loughlin, F. E., Mansfield, R. E., Vaz, P. M., McGrath, A. P., Setiyaputra, S., Gamsjaeger, R., Chen, E. S., Morris, B. J., Guss, J. M. & Mackay, J. P., 2009, In: Proceedings of the National Academy of Sciences of the United States of America. 106, 14, p. 5581-5586 6 p.

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

2008

AFM and SPR on Biological Systems: Applying Atomic Force Microscopy (AFM) and Surface Plasmon Resonance (SPR) to Biologically Important Systems

Gamsjaeger, R., 2008, Germany: VDM. 76 p.

Research output: Book/Research Report › Authored Book

NMR spectroscopy as a tool for the rapid assessment of the conformation of GST-fusion proteins

Liew, C. K., Gamsjaeger, R., Mansfield, R. E. & Mackay, J. P., 2008, In: Protein Science. 17, 9, p. 1630-1635 6 p.

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Structural and biophysical analysis of the DNA binding properties of myelin transcription factor 1

Gamsjaeger, R., Swanton, M. K., Kobus, F. J., Lehtomaki, E., Lowry, J. A., Kwan, A. H., Matthews, J. M. & Mackay, J. P., 2008, In: Journal of Biological Chemistry. 283, 8, p. 5158-5167 10 p.

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

2000

Ca2+ sensors of L-type Ca2+ channel

Romanin, C., Gamsjaeger, R., Kahr, H., Schaufler, D., Carlson, O., Abernethy, D. R. & Soldatov, N. M., 2000, In: FEBS Letters. 487, 2, p. 301-306 6 p.

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Doctor Roland Gamsjaeger

Research outputs

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