æ€¥æ€§é€’å¢žè´Ÿè·è¿åŠ¨å¯¹å°é¼ éª¨éª¼è‚ŒSODåŒå·¥é…¶æ´»æ€§å’Œmï¼²NA è¡¨è¾¾çš„å½±å“

Ping Wang; Chunguang Li; Zhengtang Qi; Shuzhe Ding

doi:10.15942/j.jcsu.2016.03.021

æ€¥æ€§é€’å¢žè´Ÿè·è¿åŠ¨å¯¹å°é¼ éª¨éª¼è‚ŒSODåŒå·¥é…¶æ´»æ€§å’Œmï¼²NA è¡¨è¾¾çš„å½±å“

Translated title of the contribution: Effect of acute incremental load exercise on SOD activity and mRNA in skeletal muscle of mice

Ping Wang, Chunguang Li, Zhengtang Qi, Shuzhe Ding

Western Sydney University

Research output: Contribution to journal › Article

Abstract

To investigate the changes of the content of NO and activity of eNOSï¼Œ the mï¼²NA expression and the activity changes of MnSOD and CuZnSOD on acute incremental exercise in skeletal muscle of mice and explore the effects of antioxidant defense systemï¼Ž Methods: thirty male ICï¼² mice were randomly divided into control group ( C) ï¼Œ45min exercise group ( E1ï¼Œn = 6) ï¼Œ90min exercise group ( E2ï¼Œn = 6) ï¼Œ120min exercise group ( E3ï¼Œn= 6) and 150min exercise group ( E4ï¼Œn = 6) ï¼Ž Mice in exercise groups were trained through treadmill exercise ( 0Ã‚Â°gradeï¼Œ5m/minï¼Œ5min /day) for 3 daysï¼Ž After adaptationï¼Œmice in exercise groups underwent treadmill exercise to the corresponding time set upï¼Ž Exercise patterns are as follows: Firstï¼Œ8ï¼Ž 2m/minï¼Œ0 gradeï¼Œ15 min ;secondï¼Œ15m/minï¼Œ5% gradeï¼Œ15min; thirdï¼Œ19ï¼Ž 3m/minï¼Œ 10% gradeï¼Ž At the end of exerciseï¼Œall mice were killed and their gastrocnemius muscle were obtainedï¼Ž Left one was used as mitochondria extractionï¼Œright one was measured by ï¼²eal ï¼ time PCï¼²ï¼Ž ï¼²esults: ( 1) Compared with control groupï¼ŒNO content significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90min( ( P ï¼œ 0ï¼Ž 01) ) ï¼Œ120min and 150min ( P ï¼œ 0ï¼Ž 05) and reached the peak at 90 minï¼Ž eNOS activity significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90min( P ï¼œ 0ï¼Ž 01) ï¼Œ120min ( P ï¼œ 0ï¼Ž 05) and reached the peak at 90minï¼Ž But the eNOS activity didn't increase in 150min group significantly ( P ï¼œ 0ï¼Ž 05) ; ( 2) MnSOD activity was significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90minï¼Œ120min and 150min ( P ï¼œ 0ï¼Ž 01) and reached the peak at 120minï¼Ž But the CuZnSOD activity didn't increase in all exercise groups significantly ( P ï¼œ 0ï¼Ž 05) ; ( 3) Compared with control groupï¼ŒMnSOD mï¼²NA significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90minï¼Œ120min and 150min ( P ï¼œ 0ï¼Ž 01) and reached the peak at 150minï¼Ž But the CuZnSOD mï¼²NA didn't increase in all exercise groups significantly ( P ï¼œ 0ï¼Ž 05) ï¼Ž Conclusion: MnSOD is the key SOD enzyme in response to the acute incremental exerciseï¼Œwhich forms a protective cycling mechanism in acute skeletal muscle exerciseï¼Ž

Translated title of the contribution	Effect of acute incremental load exercise on SOD activity and mRNA in skeletal muscle of mice
Original language	Chinese (Simplified)
Pages (from-to)	116-121
Number of pages	6
Journal	Journal of Chengdu Sport University
Volume	42
Issue number	3
DOIs	https://doi.org/10.15942/j.jcsu.2016.03.021
Publication status	Published - 2016

Keywords

antioxidants
exercise
mice as laboratory animals
musculoskeletal system

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10.15942/j.jcsu.2016.03.021

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@article{425b4cda22be444a894468676789b577,

title = "{\ae}€¥{\ae}€§{\'e}€{\textquoteright}{\aa}¢{\v z}{\`e}´Ÿ{\`e}·{\`e}¿{\aa}{\v S}¨{\aa}¯¹{\aa}°{\'e}¼ {\'e}ª¨{\'e}ª¼{\`e}‚{\OE}SOD{\aa}{\OE}{\aa}·¥{\'e}…¶{\ae}´»{\ae}€§{\aa}{\textquoteright}{\OE}m{\"i}¼²NA {\`e}¡¨{\`e}¾¾{\c c}{\v s}„{\aa}½±{\aa}“",

abstract = "To investigate the changes of the content of NO and activity of eNOS{\"i}¼{\OE} the m{\"i}¼²NA expression and the activity changes of MnSOD and CuZnSOD on acute incremental exercise in skeletal muscle of mice and explore the effects of antioxidant defense system{\"i}¼{\v Z} Methods: thirty male IC{\"i}¼² mice were randomly divided into control group ( C) {\"i}¼{\OE}45min exercise group ( E1{\"i}¼{\OE}n = 6) {\"i}¼{\OE}90min exercise group ( E2{\"i}¼{\OE}n = 6) {\"i}¼{\OE}120min exercise group ( E3{\"i}¼{\OE}n= 6) and 150min exercise group ( E4{\"i}¼{\OE}n = 6) {\"i}¼{\v Z} Mice in exercise groups were trained through treadmill exercise ( 0{\~A}‚{\^A}°grade{\"i}¼{\OE}5m/min{\"i}¼{\OE}5min /day) for 3 days{\"i}¼{\v Z} After adaptation{\"i}¼{\OE}mice in exercise groups underwent treadmill exercise to the corresponding time set up{\"i}¼{\v Z} Exercise patterns are as follows: First{\"i}¼{\OE}8{\"i}¼{\v Z} 2m/min{\"i}¼{\OE}0 grade{\"i}¼{\OE}15 min ;second{\"i}¼{\OE}15m/min{\"i}¼{\OE}5% grade{\"i}¼{\OE}15min; third{\"i}¼{\OE}19{\"i}¼{\v Z} 3m/min{\"i}¼{\OE} 10% grade{\"i}¼{\v Z} At the end of exercise{\"i}¼{\OE}all mice were killed and their gastrocnemius muscle were obtained{\"i}¼{\v Z} Left one was used as mitochondria extraction{\"i}¼{\OE}right one was measured by {\"i}¼²eal {\"i}¼ time PC{\"i}¼²{\"i}¼{\v Z} {\"i}¼²esults: ( 1) Compared with control group{\"i}¼{\OE}NO content significantly increased after 45min exercise ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 05) {\"i}¼{\OE}90min( ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 01) ) {\"i}¼{\OE}120min and 150min ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 05) and reached the peak at 90 min{\"i}¼{\v Z} eNOS activity significantly increased after 45min exercise ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 05) {\"i}¼{\OE}90min( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 01) {\"i}¼{\OE}120min ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 05) and reached the peak at 90min{\"i}¼{\v Z} But the eNOS activity didn't increase in 150min group significantly ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 05) ; ( 2) MnSOD activity was significantly increased after 45min exercise ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 05) {\"i}¼{\OE}90min{\"i}¼{\OE}120min and 150min ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 01) and reached the peak at 120min{\"i}¼{\v Z} But the CuZnSOD activity didn't increase in all exercise groups significantly ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 05) ; ( 3) Compared with control group{\"i}¼{\OE}MnSOD m{\"i}¼²NA significantly increased after 45min exercise ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 05) {\"i}¼{\OE}90min{\"i}¼{\OE}120min and 150min ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 01) and reached the peak at 150min{\"i}¼{\v Z} But the CuZnSOD m{\"i}¼²NA didn't increase in all exercise groups significantly ( P {\"i}¼{\oe} 0{\"i}¼{\v Z} 05) {\"i}¼{\v Z} Conclusion: MnSOD is the key SOD enzyme in response to the acute incremental exercise{\"i}¼{\OE}which forms a protective cycling mechanism in acute skeletal muscle exercise{\"i}¼{\v Z}",

keywords = "antioxidants, exercise, mice as laboratory animals, musculoskeletal system",

author = "Ping Wang and Chunguang Li and Zhengtang Qi and Shuzhe Ding",

year = "2016",

doi = "10.15942/j.jcsu.2016.03.021",

language = "Chinese (Simplified)",

volume = "42",

pages = "116--121",

journal = "Journal of Chengdu Sport University",

issn = "1001-9154",

publisher = "Chengdu Tiyu Xueyuan",

number = "3",

}

TY - JOUR

T1 - æ€¥æ€§é€’å¢žè´Ÿè·è¿åŠ¨å¯¹å°é¼ éª¨éª¼è‚ŒSODåŒå·¥é…¶æ´»æ€§å’Œmï¼²NA è¡¨è¾¾çš„å½±å“

AU - Wang, Ping

AU - Li, Chunguang

AU - Qi, Zhengtang

AU - Ding, Shuzhe

PY - 2016

Y1 - 2016

N2 - To investigate the changes of the content of NO and activity of eNOSï¼Œ the mï¼²NA expression and the activity changes of MnSOD and CuZnSOD on acute incremental exercise in skeletal muscle of mice and explore the effects of antioxidant defense systemï¼Ž Methods: thirty male ICï¼² mice were randomly divided into control group ( C) ï¼Œ45min exercise group ( E1ï¼Œn = 6) ï¼Œ90min exercise group ( E2ï¼Œn = 6) ï¼Œ120min exercise group ( E3ï¼Œn= 6) and 150min exercise group ( E4ï¼Œn = 6) ï¼Ž Mice in exercise groups were trained through treadmill exercise ( 0Ã‚Â°gradeï¼Œ5m/minï¼Œ5min /day) for 3 daysï¼Ž After adaptationï¼Œmice in exercise groups underwent treadmill exercise to the corresponding time set upï¼Ž Exercise patterns are as follows: Firstï¼Œ8ï¼Ž 2m/minï¼Œ0 gradeï¼Œ15 min ;secondï¼Œ15m/minï¼Œ5% gradeï¼Œ15min; thirdï¼Œ19ï¼Ž 3m/minï¼Œ 10% gradeï¼Ž At the end of exerciseï¼Œall mice were killed and their gastrocnemius muscle were obtainedï¼Ž Left one was used as mitochondria extractionï¼Œright one was measured by ï¼²eal ï¼ time PCï¼²ï¼Ž ï¼²esults: ( 1) Compared with control groupï¼ŒNO content significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90min( ( P ï¼œ 0ï¼Ž 01) ) ï¼Œ120min and 150min ( P ï¼œ 0ï¼Ž 05) and reached the peak at 90 minï¼Ž eNOS activity significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90min( P ï¼œ 0ï¼Ž 01) ï¼Œ120min ( P ï¼œ 0ï¼Ž 05) and reached the peak at 90minï¼Ž But the eNOS activity didn't increase in 150min group significantly ( P ï¼œ 0ï¼Ž 05) ; ( 2) MnSOD activity was significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90minï¼Œ120min and 150min ( P ï¼œ 0ï¼Ž 01) and reached the peak at 120minï¼Ž But the CuZnSOD activity didn't increase in all exercise groups significantly ( P ï¼œ 0ï¼Ž 05) ; ( 3) Compared with control groupï¼ŒMnSOD mï¼²NA significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90minï¼Œ120min and 150min ( P ï¼œ 0ï¼Ž 01) and reached the peak at 150minï¼Ž But the CuZnSOD mï¼²NA didn't increase in all exercise groups significantly ( P ï¼œ 0ï¼Ž 05) ï¼Ž Conclusion: MnSOD is the key SOD enzyme in response to the acute incremental exerciseï¼Œwhich forms a protective cycling mechanism in acute skeletal muscle exerciseï¼Ž

AB - To investigate the changes of the content of NO and activity of eNOSï¼Œ the mï¼²NA expression and the activity changes of MnSOD and CuZnSOD on acute incremental exercise in skeletal muscle of mice and explore the effects of antioxidant defense systemï¼Ž Methods: thirty male ICï¼² mice were randomly divided into control group ( C) ï¼Œ45min exercise group ( E1ï¼Œn = 6) ï¼Œ90min exercise group ( E2ï¼Œn = 6) ï¼Œ120min exercise group ( E3ï¼Œn= 6) and 150min exercise group ( E4ï¼Œn = 6) ï¼Ž Mice in exercise groups were trained through treadmill exercise ( 0Ã‚Â°gradeï¼Œ5m/minï¼Œ5min /day) for 3 daysï¼Ž After adaptationï¼Œmice in exercise groups underwent treadmill exercise to the corresponding time set upï¼Ž Exercise patterns are as follows: Firstï¼Œ8ï¼Ž 2m/minï¼Œ0 gradeï¼Œ15 min ;secondï¼Œ15m/minï¼Œ5% gradeï¼Œ15min; thirdï¼Œ19ï¼Ž 3m/minï¼Œ 10% gradeï¼Ž At the end of exerciseï¼Œall mice were killed and their gastrocnemius muscle were obtainedï¼Ž Left one was used as mitochondria extractionï¼Œright one was measured by ï¼²eal ï¼ time PCï¼²ï¼Ž ï¼²esults: ( 1) Compared with control groupï¼ŒNO content significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90min( ( P ï¼œ 0ï¼Ž 01) ) ï¼Œ120min and 150min ( P ï¼œ 0ï¼Ž 05) and reached the peak at 90 minï¼Ž eNOS activity significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90min( P ï¼œ 0ï¼Ž 01) ï¼Œ120min ( P ï¼œ 0ï¼Ž 05) and reached the peak at 90minï¼Ž But the eNOS activity didn't increase in 150min group significantly ( P ï¼œ 0ï¼Ž 05) ; ( 2) MnSOD activity was significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90minï¼Œ120min and 150min ( P ï¼œ 0ï¼Ž 01) and reached the peak at 120minï¼Ž But the CuZnSOD activity didn't increase in all exercise groups significantly ( P ï¼œ 0ï¼Ž 05) ; ( 3) Compared with control groupï¼ŒMnSOD mï¼²NA significantly increased after 45min exercise ( P ï¼œ 0ï¼Ž 05) ï¼Œ90minï¼Œ120min and 150min ( P ï¼œ 0ï¼Ž 01) and reached the peak at 150minï¼Ž But the CuZnSOD mï¼²NA didn't increase in all exercise groups significantly ( P ï¼œ 0ï¼Ž 05) ï¼Ž Conclusion: MnSOD is the key SOD enzyme in response to the acute incremental exerciseï¼Œwhich forms a protective cycling mechanism in acute skeletal muscle exerciseï¼Ž

KW - antioxidants

KW - exercise

KW - mice as laboratory animals

KW - musculoskeletal system

UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:51230

U2 - 10.15942/j.jcsu.2016.03.021

DO - 10.15942/j.jcsu.2016.03.021

M3 - Article

SN - 1001-9154

VL - 42

SP - 116

EP - 121

JO - Journal of Chengdu Sport University

JF - Journal of Chengdu Sport University

IS - 3

ER -

æ€¥æ€§é€’å¢žè´Ÿè·è¿åŠ¨å¯¹å°é¼ éª¨éª¼è‚ŒSODåŒå·¥é…¶æ´»æ€§å’Œmï¼²NA è¡¨è¾¾çš„å½±å“

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æ€¥æ€§é€’å¢žè´Ÿè·è¿åŠ¨å¯¹å°é¼ éª¨éª¼è‚ŒSODåŒå·¥é…¶æ´»æ€§å’Œmï¼²NA è¡¨è¾¾çš„å½±å“

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æ€¥æ€§é€’å¢žè´Ÿè·è¿åŠ¨å¯¹å°é¼ éª¨éª¼è‚ŒSODåŒå·¥é…¶æ´»æ€§å’Œmï¼²NA è¡¨è¾¾çš„å½±å“