Abstract
Objective: To explore the effect of San Huang decoction on the apoptosis of breast cancer cells and the effect on the mRNA and protein expression and function of Aurora kinase A and discuss the underlying mechanism of San Huang induced apoptosis. Methods: The inhibition of breast cancer cells proliferation was determined by CCK-8 assay. The apoptosis of breast cancer cells was detected by AnnexinV-FITC/PI Staining. The expression of mRNA of Aurora A was examined by q-PCR analysis. The expression of apoptosis-related proteins and Aurora A were determined by Western Blot analysis. Results: San Huang decoction inhibited the proliferation of breast cancer cells in a does-dependent manner (P<0.05). The effect of inhibition caused by San Huang decoction 48 hours after delivering to breast cancer cells was better than 24 hours(P<0.05), although similar as 72 hours(P>0.05). San Huang decoction was also found to induce apoptosis in both MCF-7 and MDA-MB-231 cell lines in a dose-dependent manner. Consistent with cellular results, San Huang decoction treatment significantly increased the apoptosis-related protein level of cleaved-PARP(c-PARP), cleaved-Caspase 3(c-Caspase 3) and Bax, down-regulated Bcl-2 in a does-dependent manner. Meanwhile, San Huang decoction decreased the mRNA and protein level of Aurora A and increased those of p53 in a does-dependent manner. Conclusion: San Huang decoction at the first time was able to promote the apoptosis of breast cancer cells via inducing the suppression of Aurora A.
| Translated title of the contribution | San Huang decoction induces apoptosis of breast cancer cells through regulating Aurora kinase A |
|---|---|
| Original language | Chinese (Simplified) |
| Pages (from-to) | 469-474 |
| Number of pages | 6 |
| Journal | Journal of Nanjing University of Traditional Chinese Medicine |
| Volume | 31 |
| Issue number | 5 |
| Publication status | Published - 2015 |
Keywords
- medicine, Chinese
- breast
- cancer
