通络清脑注射液对脑缺血再灌注损伤大鼠脑微透析液中两种氨基酸水平的影响

To investigate the effect of Tongluo Qingnao injection on the levels of two amino acid neurotransmitters，glutamate (Glu) and gamma-aminobutyric acid (GABA), in cerebral microdialysis samples of ischemia/reperfusion rats．Method: 24 Sprague-Dawley rats were randomly divided into 4 groups with 6 in each group, the middle cerebral artery occlusion/reperfusion (MCAO/RP) model group, the nimodipine (0.4 mg·kg－1) group, low-dose (48 mg·kg－1) group and high-dose (95 mg·kg－1) group of Tongluo Qingnao injection．Continuous cerebral microdialysis samples were acquired from the rat striatum, and the flow rate was 1 μL·min－1, each every 30 min． After 2 samples were collected, the MCAO model was established． After 90 min, the suture was removed and drugs of each group were administrated by caudal vein while reperfusion．The collection was ended 180 min after reperfusion． Glu and GABA in dialysate were pre-column derivatived and their levels were detected by an HPLC-ECD system．Result: The emission content of Glu and GABA was standardized based on data of model group．After modeling, there was a steep rise of Glu and GABA level in all groups． The emission content of Glu was significantly decreased in the Tongluo Qingnao high-dose group (327.29±127.31)% compared to the model group (1 041.08±190.57)% (P<0.05) 120 min after modeling, and the emission content of GABA was significantly decreased in the Tongluo Qingnao high-dose group (11 843.99±3952. 45)%, (4 785.80±3 051.98)% compared to the model group (35 204.61±6 919.45)%, (27 694.76±18 393.61)% (P<0.05) 120 min and 150 min after modeling, respectively．Conclusion: Tongluo Qingnao injection probably plays the neuro-protective role by inhibiting the excessive release of amino acids．