A bait-trap assay to characterize soil microbes that exhibit chemotaxis to root exudates

Katherine V. Weigh; Bruna D. Batista; Paul G. Dennis

doi:10.1007/978-1-0716-1040-4_21

A bait-trap assay to characterize soil microbes that exhibit chemotaxis to root exudates

Katherine V. Weigh, Bruna D. Batista, Paul G. Dennis

Western Sydney University

Research output: Chapter in Book / Conference Paper › Chapter

6 Citations (Scopus)

Abstract

Here, we describe a novel "bait-trap" assay, which facilitates capture of soil microorganisms that exhibit chemotaxis to chemical attractants, such as root exudates. These multi-population assemblages represent potential guilds and can be characterized using a wide-range of culture-dependent and culture-independent methods. While in this example, we use root exudates as bait, any water-soluble compound(s) could be used. Hence, the potential applications for the assay are diverse.

Original language	English
Title of host publication	Plant Microbiome: Methods and Protocols
Editors	Lilia C. Carvalhais, Paul G. Dennis
Place of Publication	U.S.
Publisher	Springer Science+Business Media
Pages	283-289
Number of pages	7
ISBN (Electronic)	9781071610404
ISBN (Print)	9781071610398
DOIs	https://doi.org/10.1007/978-1-0716-1040-4_21
Publication status	Published - 2021

Publication series

Name	Methods in Molecular Biology
Volume	2232
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Chemical language
Chemotaxis
Plant-microbe interactions
Rhizosphere
Signaling

Access to Document

10.1007/978-1-0716-1040-4_21

Cite this

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A bait-trap assay to characterize soil microbes that exhibit chemotaxis to root exudates. / Weigh, Katherine V.; Batista, Bruna D.; Dennis, Paul G.
Plant Microbiome: Methods and Protocols. ed. / Lilia C. Carvalhais; Paul G. Dennis. U.S.: Springer Science+Business Media, 2021. p. 283-289 (Methods in Molecular Biology; Vol. 2232).

Research output: Chapter in Book / Conference Paper › Chapter

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AU - Dennis, Paul G.

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KW - Chemotaxis

KW - Plant-microbe interactions

KW - Rhizosphere

KW - Signaling

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A bait-trap assay to characterize soil microbes that exhibit chemotaxis to root exudates

Abstract

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Keywords

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