A centrifugo-pneumatic cascade for fully integrated and multiplexed biological analysis

Neus Godino; Robert Gorkin; Ana V. Linares; Robert Burger; Jens Ducrée

doi:10.1109/MEMSYS.2012.6170352

A centrifugo-pneumatic cascade for fully integrated and multiplexed biological analysis

Neus Godino, Robert Gorkin, Ana V. Linares, Robert Burger, Jens Ducrée

Dublin City University

Research output: Chapter in Book / Conference Paper › Conference Paper › peer-review

3 Citations (Scopus)

Abstract

This work introduces a toolkit of structures based on centrifugo-pneumatic actuation that allows for liquid loading/transfer, metering, mixing and sedimentation in a microstructured polymer disc. This pneumatic cascade leverages full integration and automation of a range of bioassays initiated by a single sample reagent loading phase and using the same fluidic pathway for a sequence of sample and reagents. The centrifugo-pneumatic cascade also reduces manufacturing complexity as there is no need for valving based on surface coatings, geometrical microfeatures or sacrificial inlays common in other automation schemes. As a proof of concept of a homogeneous bioassay, we detect nitric oxide in whole blood by colorimetry.

Original language	English
Title of host publication	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages	989-992
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2012.6170352
Publication status	Published - 2012
Externally published	Yes
Event	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France Duration: 29 Jan 2012 → 2 Feb 2012

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Country/Territory	France
City	Paris
Period	29/01/12 → 2/02/12

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/MEMSYS.2012.6170352

Cite this

Godino, N., Gorkin, R., Linares, A. V., Burger, R., & Ducrée, J. (2012). A centrifugo-pneumatic cascade for fully integrated and multiplexed biological analysis. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (pp. 989-992). Article 6170352 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170352

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title = "A centrifugo-pneumatic cascade for fully integrated and multiplexed biological analysis",

abstract = "This work introduces a toolkit of structures based on centrifugo-pneumatic actuation that allows for liquid loading/transfer, metering, mixing and sedimentation in a microstructured polymer disc. This pneumatic cascade leverages full integration and automation of a range of bioassays initiated by a single sample reagent loading phase and using the same fluidic pathway for a sequence of sample and reagents. The centrifugo-pneumatic cascade also reduces manufacturing complexity as there is no need for valving based on surface coatings, geometrical microfeatures or sacrificial inlays common in other automation schemes. As a proof of concept of a homogeneous bioassay, we detect nitric oxide in whole blood by colorimetry.",

author = "Neus Godino and Robert Gorkin and Linares, {Ana V.} and Robert Burger and Jens Ducr{\'e}e",

year = "2012",

doi = "10.1109/MEMSYS.2012.6170352",

language = "English",

isbn = "9781467303248",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "989--992",

booktitle = "2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012",

note = "2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 ; Conference date: 29-01-2012 Through 02-02-2012",

}

Godino, N, Gorkin, R, Linares, AV, Burger, R & Ducrée, J 2012, A centrifugo-pneumatic cascade for fully integrated and multiplexed biological analysis. in 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012., 6170352, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 989-992, 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012, Paris, France, 29/01/12. https://doi.org/10.1109/MEMSYS.2012.6170352

A centrifugo-pneumatic cascade for fully integrated and multiplexed biological analysis. / Godino, Neus; Gorkin, Robert; Linares, Ana V. et al.
2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 989-992 6170352 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book / Conference Paper › Conference Paper › peer-review

TY - GEN

T1 - A centrifugo-pneumatic cascade for fully integrated and multiplexed biological analysis

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AU - Gorkin, Robert

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PY - 2012

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AB - This work introduces a toolkit of structures based on centrifugo-pneumatic actuation that allows for liquid loading/transfer, metering, mixing and sedimentation in a microstructured polymer disc. This pneumatic cascade leverages full integration and automation of a range of bioassays initiated by a single sample reagent loading phase and using the same fluidic pathway for a sequence of sample and reagents. The centrifugo-pneumatic cascade also reduces manufacturing complexity as there is no need for valving based on surface coatings, geometrical microfeatures or sacrificial inlays common in other automation schemes. As a proof of concept of a homogeneous bioassay, we detect nitric oxide in whole blood by colorimetry.

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U2 - 10.1109/MEMSYS.2012.6170352

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M3 - Conference Paper

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SN - 9781467303248

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 989

EP - 992

BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012

T2 - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012

Y2 - 29 January 2012 through 2 February 2012

ER -

Godino N, Gorkin R, Linares AV, Burger R, Ducrée J. A centrifugo-pneumatic cascade for fully integrated and multiplexed biological analysis. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 989-992. 6170352. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2012.6170352

A centrifugo-pneumatic cascade for fully integrated and multiplexed biological analysis

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