TY - JOUR
T1 - A comparative study on the inhibitory effects of different parts and chemical constituents of pomegranate on α-amylase and α-glucosidase
AU - Kam, Antony
AU - Li, Kong M.
AU - Razmovski-Naumovski, Valentina
AU - Nammi, Srinivas
AU - Shi, Jeffrey
AU - Chan, Kelvin
AU - Li, George Q.
PY - 2013
Y1 - 2013
N2 - Pomegranate has been documented for the management of diabetes in Unani and Chinese medicine. This study compared the effects of the extracts of different pomegranate parts, including juice, peels, seeds and flowers, on carbohydrate digestive enzymes (a-amylase and a-glucosidase) in vitro. The methanolic flower extract inhibited a-amylase and a-glucosidase, while the methanolic peel extract inhibited a-glucosidase selectively. The most active flower extract was subjected to water-ethyl acetate partition. The ethyl acetate fraction was more potent than the water fraction in inhibiting both enzymes. Gallic acid and ellagic acid also showed selective inhibition against a-glucosidase, and their presence in the ethyl acetate fraction was confirmed by HPLC-DAD and HPLC-HESI-MS. Our findings suggest that the inhibition of carbohydrate digestive enzymes and their phenolic content may contribute to the anti-hyperglycaemic effects of pomegranate flower and peel, and support their claims in diabetes.
AB - Pomegranate has been documented for the management of diabetes in Unani and Chinese medicine. This study compared the effects of the extracts of different pomegranate parts, including juice, peels, seeds and flowers, on carbohydrate digestive enzymes (a-amylase and a-glucosidase) in vitro. The methanolic flower extract inhibited a-amylase and a-glucosidase, while the methanolic peel extract inhibited a-glucosidase selectively. The most active flower extract was subjected to water-ethyl acetate partition. The ethyl acetate fraction was more potent than the water fraction in inhibiting both enzymes. Gallic acid and ellagic acid also showed selective inhibition against a-glucosidase, and their presence in the ethyl acetate fraction was confirmed by HPLC-DAD and HPLC-HESI-MS. Our findings suggest that the inhibition of carbohydrate digestive enzymes and their phenolic content may contribute to the anti-hyperglycaemic effects of pomegranate flower and peel, and support their claims in diabetes.
UR - http://handle.uws.edu.au:8081/1959.7/533892
U2 - 10.1002/ptr.4913
DO - 10.1002/ptr.4913
M3 - Article
SN - 0951-418X
VL - 27
SP - 1614
EP - 1620
JO - Phytotherapy Research
JF - Phytotherapy Research
IS - 11
ER -
