A mass spectrometric investigation of the ability of metal complexes to modulate transcription factor activity

Jihan Talib, Jennifer L. Beck, Thitima Urathamakul, Cuong D. Nguyen, Janice R. Aldrich-Wright, Joel P. Mackay, Stephen F. Ralph

    Research output: Contribution to journalArticlepeer-review

    Abstract

    ESI mass spectrometry was used to assess the ability of metal complexes to inhibit binding of a transcription factor to a DNA molecule containing its recognition sequence. ESI-MS showed that both [Ru(phen)₂(dppz)]²⁺ and [Pt(5,6-Me₂phen)(S,S-dach)] ²⁺ can interfere with binding of the transcription factor PU.1-DBD to a dsDNA molecule containing its consensus binding site. This further highlights the potential of metal complexes as drug leads for transcription therapy, and the potential role that ESI-MS may play as a rapid screening tool in drug development.
    Original languageEnglish
    Pages (from-to)5546-5548
    Number of pages3
    JournalChemical Communications
    Volume37
    DOIs
    Publication statusPublished - 2009

    Keywords

    • DNA, protein interactions
    • electrospray ionization mass spectrometry
    • metal complexes
    • protein binding
    • transcription factors

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