A mass spectrometric investigation of the ability of metal complexes to modulate transcription factor activity

Jihan Talib; Jennifer L. Beck; Thitima Urathamakul; Cuong D. Nguyen; Janice R. Aldrich-Wright; Joel P. Mackay; Stephen F. Ralph

doi:10.1039/b904751d

A mass spectrometric investigation of the ability of metal complexes to modulate transcription factor activity

Jihan Talib
, Jennifer L. Beck
, Thitima Urathamakul
, Cuong D. Nguyen
, Janice R. Aldrich-Wright
, Joel P. Mackay
, Stephen F. Ralph

School of Science

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

ESI mass spectrometry was used to assess the ability of metal complexes to inhibit binding of a transcription factor to a DNA molecule containing its recognition sequence. ESI-MS showed that both [Ru(phen)â‚‚(dppz)]Ã‚Â²âº and [Pt(5,6-Meâ‚‚phen)(S,S-dach)] Ã‚Â²âº can interfere with binding of the transcription factor PU.1-DBD to a dsDNA molecule containing its consensus binding site. This further highlights the potential of metal complexes as drug leads for transcription therapy, and the potential role that ESI-MS may play as a rapid screening tool in drug development.

Original language	English
Pages (from-to)	5546-5548
Number of pages	3
Journal	Chemical Communications
Volume	37
DOIs	https://doi.org/10.1039/b904751d
Publication status	Published - 2009

Keywords

DNA, protein interactions
electrospray ionization mass spectrometry
metal complexes
protein binding
transcription factors

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10.1039/b904751d

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title = "A mass spectrometric investigation of the ability of metal complexes to modulate transcription factor activity",

abstract = "ESI mass spectrometry was used to assess the ability of metal complexes to inhibit binding of a transcription factor to a DNA molecule containing its recognition sequence. ESI-MS showed that both [Ru(phen){\^a}‚‚(dppz)]{\~A}‚{\^A}²{\^a}º and [Pt(5,6-Me{\^a}‚‚phen)(S,S-dach)] {\~A}‚{\^A}²{\^a}º can interfere with binding of the transcription factor PU.1-DBD to a dsDNA molecule containing its consensus binding site. This further highlights the potential of metal complexes as drug leads for transcription therapy, and the potential role that ESI-MS may play as a rapid screening tool in drug development.",

keywords = "DNA, protein interactions, electrospray ionization mass spectrometry, metal complexes, protein binding, transcription factors",

author = "Jihan Talib and Beck, \{Jennifer L.\} and Thitima Urathamakul and Nguyen, \{Cuong D.\} and Aldrich-Wright, \{Janice R.\} and Mackay, \{Joel P.\} and Ralph, \{Stephen F.\}",

year = "2009",

doi = "10.1039/b904751d",

language = "English",

volume = "37",

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journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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T1 - A mass spectrometric investigation of the ability of metal complexes to modulate transcription factor activity

AU - Talib, Jihan

AU - Beck, Jennifer L.

AU - Urathamakul, Thitima

AU - Nguyen, Cuong D.

AU - Aldrich-Wright, Janice R.

AU - Mackay, Joel P.

AU - Ralph, Stephen F.

PY - 2009

Y1 - 2009

N2 - ESI mass spectrometry was used to assess the ability of metal complexes to inhibit binding of a transcription factor to a DNA molecule containing its recognition sequence. ESI-MS showed that both [Ru(phen)â‚‚(dppz)]Ã‚Â²âº and [Pt(5,6-Meâ‚‚phen)(S,S-dach)] Ã‚Â²âº can interfere with binding of the transcription factor PU.1-DBD to a dsDNA molecule containing its consensus binding site. This further highlights the potential of metal complexes as drug leads for transcription therapy, and the potential role that ESI-MS may play as a rapid screening tool in drug development.

AB - ESI mass spectrometry was used to assess the ability of metal complexes to inhibit binding of a transcription factor to a DNA molecule containing its recognition sequence. ESI-MS showed that both [Ru(phen)â‚‚(dppz)]Ã‚Â²âº and [Pt(5,6-Meâ‚‚phen)(S,S-dach)] Ã‚Â²âº can interfere with binding of the transcription factor PU.1-DBD to a dsDNA molecule containing its consensus binding site. This further highlights the potential of metal complexes as drug leads for transcription therapy, and the potential role that ESI-MS may play as a rapid screening tool in drug development.

KW - DNA, protein interactions

KW - electrospray ionization mass spectrometry

KW - metal complexes

KW - protein binding

KW - transcription factors

UR - http://handle.uws.edu.au:8081/1959.7/550951

U2 - 10.1039/b904751d

DO - 10.1039/b904751d

M3 - Article

C2 - 19753352

SN - 1359-7345

VL - 37

SP - 5546

EP - 5548

JO - Chemical Communications

JF - Chemical Communications

ER -

A mass spectrometric investigation of the ability of metal complexes to modulate transcription factor activity

Abstract

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