A potentially new class of redox-sensitive CEST agents : preliminary Thiol-CEST studies with glutathione and cysteine

Johnny Chen; Nirbhay N. Yadav; T. Stait-Gardner; Abhishek Gupta; William S. Price; Gang Zheng

A potentially new class of redox-sensitive CEST agents : preliminary Thiol-CEST studies with glutathione and cysteine

Johnny Chen, Nirbhay N. Yadav, T. Stait-Gardner, Abhishek Gupta, William S. Price, Gang Zheng

Research output: Chapter in Book / Conference Paper › Conference Paper

Abstract

In this study, we show that thiol-water proton exchange can generate MRI contrast by performing proton chemical exchange saturation transfer (1H-CEST) experiments on glutathione (GSH) and cysteine (Cys). The thiol proton exchange was quantified at various pHs, with Cys thiol exhibiting faster and more base-catalyzed exchange than GSH. However, both GSH and Cys thiol exchange were too fast to generate contrast at physiological pH. Potential applications of thiol compounds as redox-sensitive CEST agents are suggested.

Original language	English
Title of host publication	27th International Society for Magnetic Resonance in Medicine (ISMRM 2019) Annual Meeting and Exhibition, Montreal, QC, Canada, 11-16 May, 2019
Publisher	International Society for Magnetic Resonance in Medicine
Number of pages	5
Publication status	Published - 2019
Event	International Society for Magnetic Resonance in Medicine - Duration: 1 Jan 2019 → …

Conference

Conference	International Society for Magnetic Resonance in Medicine
Period	1/01/19 → …

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Chen, J., Yadav, N. N., Stait-Gardner, T., Gupta, A., Price, W. S., & Zheng, G. (2019). A potentially new class of redox-sensitive CEST agents : preliminary Thiol-CEST studies with glutathione and cysteine. In 27th International Society for Magnetic Resonance in Medicine (ISMRM 2019) Annual Meeting and Exhibition, Montreal, QC, Canada, 11-16 May, 2019 International Society for Magnetic Resonance in Medicine.

Chen, Johnny ; Yadav, Nirbhay N. ; Stait-Gardner, T. et al. / A potentially new class of redox-sensitive CEST agents : preliminary Thiol-CEST studies with glutathione and cysteine. 27th International Society for Magnetic Resonance in Medicine (ISMRM 2019) Annual Meeting and Exhibition, Montreal, QC, Canada, 11-16 May, 2019. International Society for Magnetic Resonance in Medicine, 2019.

@inproceedings{fb0e6738e2ae4dfca6f3b66d535605cc,

title = "A potentially new class of redox-sensitive CEST agents : preliminary Thiol-CEST studies with glutathione and cysteine",

abstract = "In this study, we show that thiol-water proton exchange can generate MRI contrast by performing proton chemical exchange saturation transfer (1H-CEST) experiments on glutathione (GSH) and cysteine (Cys). The thiol proton exchange was quantified at various pHs, with Cys thiol exhibiting faster and more base-catalyzed exchange than GSH. However, both GSH and Cys thiol exchange were too fast to generate contrast at physiological pH. Potential applications of thiol compounds as redox-sensitive CEST agents are suggested.",

author = "Johnny Chen and Yadav, {Nirbhay N.} and T. Stait-Gardner and Abhishek Gupta and Price, {William S.} and Gang Zheng",

year = "2019",

language = "English",

booktitle = "27th International Society for Magnetic Resonance in Medicine (ISMRM 2019) Annual Meeting and Exhibition, Montreal, QC, Canada, 11-16 May, 2019",

publisher = "International Society for Magnetic Resonance in Medicine",

note = "International Society for Magnetic Resonance in Medicine ; Conference date: 01-01-2019",

}

Chen, J, Yadav, NN, Stait-Gardner, T, Gupta, A, Price, WS & Zheng, G 2019, A potentially new class of redox-sensitive CEST agents : preliminary Thiol-CEST studies with glutathione and cysteine. in 27th International Society for Magnetic Resonance in Medicine (ISMRM 2019) Annual Meeting and Exhibition, Montreal, QC, Canada, 11-16 May, 2019. International Society for Magnetic Resonance in Medicine, International Society for Magnetic Resonance in Medicine, 1/01/19.

A potentially new class of redox-sensitive CEST agents : preliminary Thiol-CEST studies with glutathione and cysteine. / Chen, Johnny; Yadav, Nirbhay N.; Stait-Gardner, T. et al.
27th International Society for Magnetic Resonance in Medicine (ISMRM 2019) Annual Meeting and Exhibition, Montreal, QC, Canada, 11-16 May, 2019. International Society for Magnetic Resonance in Medicine, 2019.

Research output: Chapter in Book / Conference Paper › Conference Paper

TY - GEN

T1 - A potentially new class of redox-sensitive CEST agents : preliminary Thiol-CEST studies with glutathione and cysteine

AU - Chen, Johnny

AU - Yadav, Nirbhay N.

AU - Stait-Gardner, T.

AU - Gupta, Abhishek

AU - Price, William S.

AU - Zheng, Gang

PY - 2019

Y1 - 2019

N2 - In this study, we show that thiol-water proton exchange can generate MRI contrast by performing proton chemical exchange saturation transfer (1H-CEST) experiments on glutathione (GSH) and cysteine (Cys). The thiol proton exchange was quantified at various pHs, with Cys thiol exhibiting faster and more base-catalyzed exchange than GSH. However, both GSH and Cys thiol exchange were too fast to generate contrast at physiological pH. Potential applications of thiol compounds as redox-sensitive CEST agents are suggested.

AB - In this study, we show that thiol-water proton exchange can generate MRI contrast by performing proton chemical exchange saturation transfer (1H-CEST) experiments on glutathione (GSH) and cysteine (Cys). The thiol proton exchange was quantified at various pHs, with Cys thiol exhibiting faster and more base-catalyzed exchange than GSH. However, both GSH and Cys thiol exchange were too fast to generate contrast at physiological pH. Potential applications of thiol compounds as redox-sensitive CEST agents are suggested.

UR - https://hdl.handle.net/1959.7/uws:61218

M3 - Conference Paper

BT - 27th International Society for Magnetic Resonance in Medicine (ISMRM 2019) Annual Meeting and Exhibition, Montreal, QC, Canada, 11-16 May, 2019

PB - International Society for Magnetic Resonance in Medicine

T2 - International Society for Magnetic Resonance in Medicine

Y2 - 1 January 2019

ER -

Chen J, Yadav NN, Stait-Gardner T, Gupta A, Price WS , Zheng G. A potentially new class of redox-sensitive CEST agents : preliminary Thiol-CEST studies with glutathione and cysteine. In 27th International Society for Magnetic Resonance in Medicine (ISMRM 2019) Annual Meeting and Exhibition, Montreal, QC, Canada, 11-16 May, 2019. International Society for Magnetic Resonance in Medicine. 2019

A potentially new class of redox-sensitive CEST agents : preliminary Thiol-CEST studies with glutathione and cysteine

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