A rapid screening analysis of antioxidant compounds in native Australian food plants using multiplexed detection with active flow technology columns

Emmanuel Janaka Rochana Rupesinghe; Andrew Jones; Ross Andrew Shalliker; Sercan Pravadali-Cekic

doi:10.3390/molecules21010118

A rapid screening analysis of antioxidant compounds in native Australian food plants using multiplexed detection with active flow technology columns

Emmanuel Janaka Rochana Rupesinghe, Andrew Jones, Ross Andrew Shalliker, Sercan Pravadali-Cekic

Research output: Contribution to journal › Article › peer-review

Abstract

Conventional techniques for identifying antioxidant and phenolic compounds in native Australian food plants are laborious and time-consuming. Here, we present a multiplexed detection technique that reduces analysis time without compromising separation performance. This technique is achieved using Active Flow Technology-Parallel Segmented Flow (AFT-PSF) columns. Extracts from cinnamon myrtle (Backhousia myrtifolia) and lemon myrtle (Backhousia citriodora) leaves were analysed via multiplexed detection using an AFT-PSF column with underivatised UV-VIS, mass spectroscopy (MS), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) derivatisation for antioxidants as detection methods. A number of antioxidant compounds were detected in the extracts of each leaf extract.

Original language	English
Article number	118
Number of pages	14
Journal	Molecules
Volume	21
Issue number	1
DOIs	https://doi.org/10.3390/molecules21010118
Publication status	Published - 2016

Open Access - Access Right Statement

© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

Keywords

Australia
active flow technology
antioxidants
high performance liquid chromatography
wild plants, edible

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10.3390/molecules21010118

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T1 - A rapid screening analysis of antioxidant compounds in native Australian food plants using multiplexed detection with active flow technology columns

AU - Rupesinghe, Emmanuel Janaka Rochana

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AU - Shalliker, Ross Andrew

AU - Pravadali-Cekic, Sercan

PY - 2016

Y1 - 2016

N2 - Conventional techniques for identifying antioxidant and phenolic compounds in native Australian food plants are laborious and time-consuming. Here, we present a multiplexed detection technique that reduces analysis time without compromising separation performance. This technique is achieved using Active Flow Technology-Parallel Segmented Flow (AFT-PSF) columns. Extracts from cinnamon myrtle (Backhousia myrtifolia) and lemon myrtle (Backhousia citriodora) leaves were analysed via multiplexed detection using an AFT-PSF column with underivatised UV-VIS, mass spectroscopy (MS), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) derivatisation for antioxidants as detection methods. A number of antioxidant compounds were detected in the extracts of each leaf extract.

AB - Conventional techniques for identifying antioxidant and phenolic compounds in native Australian food plants are laborious and time-consuming. Here, we present a multiplexed detection technique that reduces analysis time without compromising separation performance. This technique is achieved using Active Flow Technology-Parallel Segmented Flow (AFT-PSF) columns. Extracts from cinnamon myrtle (Backhousia myrtifolia) and lemon myrtle (Backhousia citriodora) leaves were analysed via multiplexed detection using an AFT-PSF column with underivatised UV-VIS, mass spectroscopy (MS), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) derivatisation for antioxidants as detection methods. A number of antioxidant compounds were detected in the extracts of each leaf extract.

KW - Australia

KW - active flow technology

KW - antioxidants

KW - high performance liquid chromatography

KW - wild plants, edible

UR - http://handle.uws.edu.au:8081/1959.7/uws:33631

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DO - 10.3390/molecules21010118

M3 - Article

SN - 1420-3049

VL - 21

JO - Molecules

JF - Molecules

IS - 1

M1 - 118

ER -

A rapid screening analysis of antioxidant compounds in native Australian food plants using multiplexed detection with active flow technology columns

Abstract

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