A review on multiplexed detection using active flow technology columns

The analysis and characterisation of complex samples of natural origin can be an arduous and time consuming task, especially under uni-dimensional HPLC conditions. There are numerous detectors that have been applied to the analysis of natural origin samples, however, there is no universal detector can that provide a complete analysis of complex samples. Thus, to reduce sample complexity multi-detection analysis for multidimensional sample information is sought. A new form of column technology known as Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode provides a platform for multiplexed detection. Multiplexing via AFT-PSF allows the simultaneous use of up to four destructive and non-destructive detectors whilst maintaining chromatographic performance to achieve a comprehensive yet efficient characterisation of a complex sample. This review discusses the various available detectors used in HPLC, the advantages and disadvantages of the various multi-detection systems and the considerations that must be made when establishing a multiplexed system with AFT-PSF.