A simple and inexpensive method for preparing erythrocyte membranes by filtration through a hollow-fiber system

William S. Price; Basil Roufogalis; Philip W. Kuchel

doi:10.1016/0003-2697(89)90223-6

A simple and inexpensive method for preparing erythrocyte membranes by filtration through a hollow-fiber system

William S. Price, Basil Roufogalis, Philip W. Kuchel

University of Sydney

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

An efficient, simple method for preparing biologically active erythrocyte membranes is described. The semiautomated procedure involves circulating hemolysate mixture through a hollow-fiber system, thereby filtering off intracellular components leaving a high yield of washed ghosts. The prepared ghosts exhibit cation-stimulated ATPase activities comparable to those of ghosts prepared by traditional methods. Electron micrographs revealed that the filtration isolation caused less shearing of the membranes than procedures based solely on centrifugation.

Original language	English
Pages (from-to)	190-193
Number of pages	4
Journal	Analytical Biochemistry
Volume	179
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(89)90223-6
Publication status	Published - 15 May 1989
Externally published	Yes

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10.1016/0003-2697(89)90223-6

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abstract = "An efficient, simple method for preparing biologically active erythrocyte membranes is described. The semiautomated procedure involves circulating hemolysate mixture through a hollow-fiber system, thereby filtering off intracellular components leaving a high yield of washed ghosts. The prepared ghosts exhibit cation-stimulated ATPase activities comparable to those of ghosts prepared by traditional methods. Electron micrographs revealed that the filtration isolation caused less shearing of the membranes than procedures based solely on centrifugation.",

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AB - An efficient, simple method for preparing biologically active erythrocyte membranes is described. The semiautomated procedure involves circulating hemolysate mixture through a hollow-fiber system, thereby filtering off intracellular components leaving a high yield of washed ghosts. The prepared ghosts exhibit cation-stimulated ATPase activities comparable to those of ghosts prepared by traditional methods. Electron micrographs revealed that the filtration isolation caused less shearing of the membranes than procedures based solely on centrifugation.

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A simple and inexpensive method for preparing erythrocyte membranes by filtration through a hollow-fiber system

Abstract

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