A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells

Chitra Umala Dewi, Michele Mason, Tzipi Cohen-Hyams, Murray C. Killingsworth, David G. Harman, Vikkitharan Gnanasambandapillai, Liwan Liyanage, Michael D. O'Connor

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Here we describe a modified method for harvesting tens-of-millions of human lens epithelial-like cells from differentiated pluripotent stem cell cultures. To assess the utility of this method, we analysed the lens cell population via: light microscopy; single cell RNA-sequencing and gene ontology analyses; formation of light-focusing micro-lenses; mass spectrometry; and electron microscopy. Both individually and collectively, the data indicate this simplified harvesting method provides a large-scale source of stem cell-derived lens cells and micro-lenses for investigating human lens and cataract formation.
Original languageEnglish
Article number108317
Number of pages9
JournalExperimental Eye Research
Volume202
DOIs
Publication statusPublished - 2021

Keywords

  • bioinformatics
  • cataract
  • epithelial cells
  • eye
  • human beings

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