Abstract
Here we describe a modified method for harvesting tens-of-millions of human lens epithelial-like cells from differentiated pluripotent stem cell cultures. To assess the utility of this method, we analysed the lens cell population via: light microscopy; single cell RNA-sequencing and gene ontology analyses; formation of light-focusing micro-lenses; mass spectrometry; and electron microscopy. Both individually and collectively, the data indicate this simplified harvesting method provides a large-scale source of stem cell-derived lens cells and micro-lenses for investigating human lens and cataract formation.
Original language | English |
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Article number | 108317 |
Number of pages | 9 |
Journal | Experimental Eye Research |
Volume | 202 |
DOIs | |
Publication status | Published - 2021 |
Keywords
- bioinformatics
- cataract
- epithelial cells
- eye
- human beings