A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells

Chitra Umala Dewi; Michele Mason; Tzipi Cohen-Hyams; Murray C. Killingsworth; David G. Harman; Vikkitharan Gnanasambandapillai; Liwan Liyanage; Michael D. O'Connor

doi:10.1016/j.exer.2020.108317

A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells

Chitra Umala Dewi, Michele Mason, Tzipi Cohen-Hyams, Murray C. Killingsworth, David G. Harman, Vikkitharan Gnanasambandapillai, Liwan Liyanage, Michael D. O'Connor

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Here we describe a modified method for harvesting tens-of-millions of human lens epithelial-like cells from differentiated pluripotent stem cell cultures. To assess the utility of this method, we analysed the lens cell population via: light microscopy; single cell RNA-sequencing and gene ontology analyses; formation of light-focusing micro-lenses; mass spectrometry; and electron microscopy. Both individually and collectively, the data indicate this simplified harvesting method provides a large-scale source of stem cell-derived lens cells and micro-lenses for investigating human lens and cataract formation.

Original language	English
Article number	108317
Number of pages	9
Journal	Experimental Eye Research
Volume	202
DOIs	https://doi.org/10.1016/j.exer.2020.108317
Publication status	Published - Jan 2021

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

bioinformatics
cataract
epithelial cells
eye
human beings

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.exer.2020.108317

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abstract = "Here we describe a modified method for harvesting tens-of-millions of human lens epithelial-like cells from differentiated pluripotent stem cell cultures. To assess the utility of this method, we analysed the lens cell population via: light microscopy; single cell RNA-sequencing and gene ontology analyses; formation of light-focusing micro-lenses; mass spectrometry; and electron microscopy. Both individually and collectively, the data indicate this simplified harvesting method provides a large-scale source of stem cell-derived lens cells and micro-lenses for investigating human lens and cataract formation.",

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doi = "10.1016/j.exer.2020.108317",

language = "English",

volume = "202",

journal = "Experimental Eye Research",

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T1 - A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells

AU - Dewi, Chitra Umala

AU - Mason, Michele

AU - Cohen-Hyams, Tzipi

AU - Killingsworth, Murray C.

AU - Harman, David G.

AU - Gnanasambandapillai, Vikkitharan

AU - Liyanage, Liwan

AU - O'Connor, Michael D.

PY - 2021/1

Y1 - 2021/1

N2 - Here we describe a modified method for harvesting tens-of-millions of human lens epithelial-like cells from differentiated pluripotent stem cell cultures. To assess the utility of this method, we analysed the lens cell population via: light microscopy; single cell RNA-sequencing and gene ontology analyses; formation of light-focusing micro-lenses; mass spectrometry; and electron microscopy. Both individually and collectively, the data indicate this simplified harvesting method provides a large-scale source of stem cell-derived lens cells and micro-lenses for investigating human lens and cataract formation.

AB - Here we describe a modified method for harvesting tens-of-millions of human lens epithelial-like cells from differentiated pluripotent stem cell cultures. To assess the utility of this method, we analysed the lens cell population via: light microscopy; single cell RNA-sequencing and gene ontology analyses; formation of light-focusing micro-lenses; mass spectrometry; and electron microscopy. Both individually and collectively, the data indicate this simplified harvesting method provides a large-scale source of stem cell-derived lens cells and micro-lenses for investigating human lens and cataract formation.

KW - bioinformatics

KW - cataract

KW - epithelial cells

KW - eye

KW - human beings

UR - https://hdl.handle.net/1959.7/uws:59051

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DO - 10.1016/j.exer.2020.108317

M3 - Article

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SN - 0014-4835

VL - 202

JO - Experimental Eye Research

JF - Experimental Eye Research

M1 - 108317

ER -

A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells

Abstract

Bibliographical note

Keywords

UN SDGs

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