A structural perspective on the regulation of human single-stranded DNA binding protein 1 (hSSB1, OBFC2B) function in DNA repair

Teegan Lawson; Serene El-Kamand; Ruvini Kariawasam; Derek J. Richard; Liza Cubeddu; Roland Gamsjaeger

doi:10.1016/j.csbj.2019.03.014

A structural perspective on the regulation of human single-stranded DNA binding protein 1 (hSSB1, OBFC2B) function in DNA repair

Teegan Lawson, Serene El-Kamand, Ruvini Kariawasam, Derek J. Richard, Liza Cubeddu, Roland Gamsjaeger

Western Sydney University

Research output: Contribution to journal › Article › peer-review

Abstract

Single-stranded DNA binding (SSB) proteins are essential to protect singe-stranded DNA (ssDNA) that exists as a result of several important DNA repair pathways in living cells. In humans, besides the well-characterised Replication Protein A (RPA) we have described another SSB termed human SSB1 (hSSB1, OBFC2B) and have shown that this protein is an important player in the maintenance of the genome. In this review we define the structural and biophysical details of how hSSB1 interacts with both DNA and other essential proteins. While the presence of the oligonucleotide/oligosaccharide (OB) domain ensures ssDNA binding by hSSB1, it has also been shown to self-oligomerise as well as interact with and being modified by several proteins highlighting the versatility that hSSB1 displays in the context of DNA repair. A detailed structural understanding of these processes will likely lead to the designs of tailored hSSB1 inhibitors as anti-cancer drugs in the near future.

Original language	English
Pages (from-to)	441-446
Number of pages	6
Journal	Computational and Structural Biotechnology Journal
Volume	17
DOIs	https://doi.org/10.1016/j.csbj.2019.03.014
Publication status	Published - 2019

Open Access - Access Right Statement

© 2019 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)

Keywords

DNA repair
DNA-binding proteins
antineoplastic agents
cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.csbj.2019.03.014

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title = "A structural perspective on the regulation of human single-stranded DNA binding protein 1 (hSSB1, OBFC2B) function in DNA repair",

abstract = "Single-stranded DNA binding (SSB) proteins are essential to protect singe-stranded DNA (ssDNA) that exists as a result of several important DNA repair pathways in living cells. In humans, besides the well-characterised Replication Protein A (RPA) we have described another SSB termed human SSB1 (hSSB1, OBFC2B) and have shown that this protein is an important player in the maintenance of the genome. In this review we define the structural and biophysical details of how hSSB1 interacts with both DNA and other essential proteins. While the presence of the oligonucleotide/oligosaccharide (OB) domain ensures ssDNA binding by hSSB1, it has also been shown to self-oligomerise as well as interact with and being modified by several proteins highlighting the versatility that hSSB1 displays in the context of DNA repair. A detailed structural understanding of these processes will likely lead to the designs of tailored hSSB1 inhibitors as anti-cancer drugs in the near future.",

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author = "Teegan Lawson and Serene El-Kamand and Ruvini Kariawasam and Richard, {Derek J.} and Liza Cubeddu and Roland Gamsjaeger",

year = "2019",

doi = "10.1016/j.csbj.2019.03.014",

language = "English",

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pages = "441--446",

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T1 - A structural perspective on the regulation of human single-stranded DNA binding protein 1 (hSSB1, OBFC2B) function in DNA repair

AU - Lawson, Teegan

AU - El-Kamand, Serene

AU - Kariawasam, Ruvini

AU - Richard, Derek J.

AU - Cubeddu, Liza

AU - Gamsjaeger, Roland

PY - 2019

Y1 - 2019

N2 - Single-stranded DNA binding (SSB) proteins are essential to protect singe-stranded DNA (ssDNA) that exists as a result of several important DNA repair pathways in living cells. In humans, besides the well-characterised Replication Protein A (RPA) we have described another SSB termed human SSB1 (hSSB1, OBFC2B) and have shown that this protein is an important player in the maintenance of the genome. In this review we define the structural and biophysical details of how hSSB1 interacts with both DNA and other essential proteins. While the presence of the oligonucleotide/oligosaccharide (OB) domain ensures ssDNA binding by hSSB1, it has also been shown to self-oligomerise as well as interact with and being modified by several proteins highlighting the versatility that hSSB1 displays in the context of DNA repair. A detailed structural understanding of these processes will likely lead to the designs of tailored hSSB1 inhibitors as anti-cancer drugs in the near future.

AB - Single-stranded DNA binding (SSB) proteins are essential to protect singe-stranded DNA (ssDNA) that exists as a result of several important DNA repair pathways in living cells. In humans, besides the well-characterised Replication Protein A (RPA) we have described another SSB termed human SSB1 (hSSB1, OBFC2B) and have shown that this protein is an important player in the maintenance of the genome. In this review we define the structural and biophysical details of how hSSB1 interacts with both DNA and other essential proteins. While the presence of the oligonucleotide/oligosaccharide (OB) domain ensures ssDNA binding by hSSB1, it has also been shown to self-oligomerise as well as interact with and being modified by several proteins highlighting the versatility that hSSB1 displays in the context of DNA repair. A detailed structural understanding of these processes will likely lead to the designs of tailored hSSB1 inhibitors as anti-cancer drugs in the near future.

KW - DNA repair

KW - DNA-binding proteins

KW - antineoplastic agents

KW - cancer

UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:51443

U2 - 10.1016/j.csbj.2019.03.014

DO - 10.1016/j.csbj.2019.03.014

M3 - Article

SN - 2001-0370

VL - 17

SP - 441

EP - 446

JO - Computational and Structural Biotechnology Journal

JF - Computational and Structural Biotechnology Journal

ER -

A structural perspective on the regulation of human single-stranded DNA binding protein 1 (hSSB1, OBFC2B) function in DNA repair

Abstract

Open Access - Access Right Statement

Keywords

UN SDGs

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