A study of Pt(II)-phenanthroline complex interactions with double-stranded and G-quadruplex DNA by ESI-MS, circular dichroism, and computational docking

Dale L. Ang; Celine Kelso; Jennifer L. Beck; Stephen F. Ralph; David G. Harman; Janice R. Aldrich-Wright

doi:10.1007/s00775-020-01773-4

A study of Pt(II)-phenanthroline complex interactions with double-stranded and G-quadruplex DNA by ESI-MS, circular dichroism, and computational docking

Dale L. Ang
, Celine Kelso
, Jennifer L. Beck
, Stephen F. Ralph
, David G. Harman
, Janice R. Aldrich-Wright

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Abstract: The binding interactions of a series of square-planar platinum(II)–phenanthroline complexes of the type [Pt(P_L)(A_L)]²⁺ [where P_L = variously methyl-substituted 1,10-phenanthroline (phen) and A_L = ethane-1,2-diamine (en)] were assessed with a G-quadruplex DNA (5′-TTG GGG GT-3′, G4DNA) and a double-stranded DNA (5′-CGC GAA TTC GCG-3′, dsDNA) sequence by ESI–MS. The results indicate a strong correlation between G4DNA affinity and increasing phenanthroline methyl substitution. Circular dichroism (CD) spectroscopy and molecular docking studies also support the finding that increased substitution of the phenanthroline ligand increased selectivity for G4DNA. Graphic abstract: ESI-MS was used to probe the interaction of a range of square-planar Pt(II)–phenanthroline complexes with double-stranded and G-quadruplex DNA. [Figure not available: see fulltext.].

Original language	English
Pages (from-to)	429-440
Number of pages	12
Journal	Journal of Biological Inorganic Chemistry
Volume	25
Issue number	3
DOIs	https://doi.org/10.1007/s00775-020-01773-4
Publication status	Published - 1 May 2020

Bibliographical note

Publisher Copyright:
© 2020, Society for Biological Inorganic Chemistry (SBIC).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

antineoplastic agents
mass spectrometry
platinum

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10.1007/s00775-020-01773-4

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@article{33358fcabf654dd7877d10967fde8639,

title = "A study of Pt(II)-phenanthroline complex interactions with double-stranded and G-quadruplex DNA by ESI-MS, circular dichroism, and computational docking",

abstract = "Abstract: The binding interactions of a series of square-planar platinum(II){\textendash}phenanthroline complexes of the type [Pt(PL)(AL)]2+ [where PL = variously methyl-substituted 1,10-phenanthroline (phen) and AL = ethane-1,2-diamine (en)] were assessed with a G-quadruplex DNA (5′-TTG GGG GT-3′, G4DNA) and a double-stranded DNA (5′-CGC GAA TTC GCG-3′, dsDNA) sequence by ESI{\textendash}MS. The results indicate a strong correlation between G4DNA affinity and increasing phenanthroline methyl substitution. Circular dichroism (CD) spectroscopy and molecular docking studies also support the finding that increased substitution of the phenanthroline ligand increased selectivity for G4DNA. Graphic abstract: ESI-MS was used to probe the interaction of a range of square-planar Pt(II){\textendash}phenanthroline complexes with double-stranded and G-quadruplex DNA. [Figure not available: see fulltext.].",

keywords = "antineoplastic agents, mass spectrometry, platinum",

author = "Ang, \{Dale L.\} and Celine Kelso and Beck, \{Jennifer L.\} and Ralph, \{Stephen F.\} and Harman, \{David G.\} and Aldrich-Wright, \{Janice R.\}",

note = "Publisher Copyright: {\textcopyright} 2020, Society for Biological Inorganic Chemistry (SBIC).",

year = "2020",

month = may,

day = "1",

doi = "10.1007/s00775-020-01773-4",

language = "English",

volume = "25",

pages = "429--440",

journal = "Journal of Biological Inorganic Chemistry",

issn = "0949-8257",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "3",

}

TY - JOUR

T1 - A study of Pt(II)-phenanthroline complex interactions with double-stranded and G-quadruplex DNA by ESI-MS, circular dichroism, and computational docking

AU - Ang, Dale L.

AU - Kelso, Celine

AU - Beck, Jennifer L.

AU - Ralph, Stephen F.

AU - Harman, David G.

AU - Aldrich-Wright, Janice R.

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Abstract: The binding interactions of a series of square-planar platinum(II)–phenanthroline complexes of the type [Pt(PL)(AL)]2+ [where PL = variously methyl-substituted 1,10-phenanthroline (phen) and AL = ethane-1,2-diamine (en)] were assessed with a G-quadruplex DNA (5′-TTG GGG GT-3′, G4DNA) and a double-stranded DNA (5′-CGC GAA TTC GCG-3′, dsDNA) sequence by ESI–MS. The results indicate a strong correlation between G4DNA affinity and increasing phenanthroline methyl substitution. Circular dichroism (CD) spectroscopy and molecular docking studies also support the finding that increased substitution of the phenanthroline ligand increased selectivity for G4DNA. Graphic abstract: ESI-MS was used to probe the interaction of a range of square-planar Pt(II)–phenanthroline complexes with double-stranded and G-quadruplex DNA. [Figure not available: see fulltext.].

AB - Abstract: The binding interactions of a series of square-planar platinum(II)–phenanthroline complexes of the type [Pt(PL)(AL)]2+ [where PL = variously methyl-substituted 1,10-phenanthroline (phen) and AL = ethane-1,2-diamine (en)] were assessed with a G-quadruplex DNA (5′-TTG GGG GT-3′, G4DNA) and a double-stranded DNA (5′-CGC GAA TTC GCG-3′, dsDNA) sequence by ESI–MS. The results indicate a strong correlation between G4DNA affinity and increasing phenanthroline methyl substitution. Circular dichroism (CD) spectroscopy and molecular docking studies also support the finding that increased substitution of the phenanthroline ligand increased selectivity for G4DNA. Graphic abstract: ESI-MS was used to probe the interaction of a range of square-planar Pt(II)–phenanthroline complexes with double-stranded and G-quadruplex DNA. [Figure not available: see fulltext.].

KW - antineoplastic agents

KW - mass spectrometry

KW - platinum

UR - http://hdl.handle.net/1959.7/uws:55228

U2 - 10.1007/s00775-020-01773-4

DO - 10.1007/s00775-020-01773-4

M3 - Article

C2 - 32219553

SN - 0949-8257

VL - 25

SP - 429

EP - 440

JO - Journal of Biological Inorganic Chemistry

JF - Journal of Biological Inorganic Chemistry

IS - 3

ER -

A study of Pt(II)-phenanthroline complex interactions with double-stranded and G-quadruplex DNA by ESI-MS, circular dichroism, and computational docking

Abstract

Bibliographical note

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Keywords

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