Aequorea's secrets revealed : new fluorescent proteins with unique properties for bioimaging and biosensing

Gerard G. Lambert; Hadrien Depernet; Guillaume Gotthard; Darrin T. Schultz; Isabelle Navizet; Talley Lambert; Stephen R. Adams; Albertina Torreblanca-Zanca; Meihua Chu; Daphne S. Bindels; Vincent Levesque; Jennifer Nero Moffatt; Anya Salih; Antoine Royant; Nathan C. Shaner

doi:10.1371/journal.pbio.3000936

Aequorea's secrets revealed : new fluorescent proteins with unique properties for bioimaging and biosensing

Gerard G. Lambert, Hadrien Depernet, Guillaume Gotthard, Darrin T. Schultz, Isabelle Navizet, Talley Lambert, Stephen R. Adams, Albertina Torreblanca-Zanca, Meihua Chu, Daphne S. Bindels, Vincent Levesque, Jennifer Nero Moffatt, Anya Salih, Antoine Royant, Nathan C. Shaner

Western Sydney University

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

Using mRNA sequencing and de novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent from A. victoria green fluorescent protein (avGFP). Among these FPs are the brightest green fluorescent protein (GFP) homolog yet characterized and a reversibly photochromic FP that responds to UV and blue light. Beyond green emitters, Aequorea species express purple- and blue-pigmented chromoproteins (CPs) with absorbances ranging from green to far-red, including 2 that are photoconvertible. X-ray crystallography revealed that Aequorea CPs contain a chemically novel chromophore with an unexpected crosslink to the main polypeptide chain. Because of the unique attributes of several of these newly discovered FPs, we expect that Aequorea will, once again, give rise to an entirely new generation of useful probes for bioimaging and biosensing.

Original language	English
Article number	e3000936
Number of pages	22
Journal	PLoS Biology
Volume	18
Issue number	11
DOIs	https://doi.org/10.1371/journal.pbio.3000936
Publication status	Published - 2 Nov 2020

Bibliographical note

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© 2020 Lambert et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Open Access - Access Right Statement

© 2020 Lambert et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Lambert, G. G., Depernet, H., Gotthard, G., Schultz, D. T., Navizet, I., Lambert, T., Adams, S. R., Torreblanca-Zanca, A., Chu, M., Bindels, D. S., Levesque, V., Moffatt, J. N., Salih, A., Royant, A., & Shaner, N. C. (2020). Aequorea's secrets revealed : new fluorescent proteins with unique properties for bioimaging and biosensing. PLoS Biology, 18(11), Article e3000936. https://doi.org/10.1371/journal.pbio.3000936

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title = "Aequorea's secrets revealed : new fluorescent proteins with unique properties for bioimaging and biosensing",

abstract = "Using mRNA sequencing and de novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent from A. victoria green fluorescent protein (avGFP). Among these FPs are the brightest green fluorescent protein (GFP) homolog yet characterized and a reversibly photochromic FP that responds to UV and blue light. Beyond green emitters, Aequorea species express purple- and blue-pigmented chromoproteins (CPs) with absorbances ranging from green to far-red, including 2 that are photoconvertible. X-ray crystallography revealed that Aequorea CPs contain a chemically novel chromophore with an unexpected crosslink to the main polypeptide chain. Because of the unique attributes of several of these newly discovered FPs, we expect that Aequorea will, once again, give rise to an entirely new generation of useful probes for bioimaging and biosensing.",

author = "Lambert, {Gerard G.} and Hadrien Depernet and Guillaume Gotthard and Schultz, {Darrin T.} and Isabelle Navizet and Talley Lambert and Adams, {Stephen R.} and Albertina Torreblanca-Zanca and Meihua Chu and Bindels, {Daphne S.} and Vincent Levesque and Moffatt, {Jennifer Nero} and Anya Salih and Antoine Royant and Shaner, {Nathan C.}",

note = "Publisher Copyright: {\textcopyright} 2020 Lambert et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

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Lambert, GG, Depernet, H, Gotthard, G, Schultz, DT, Navizet, I, Lambert, T, Adams, SR, Torreblanca-Zanca, A, Chu, M, Bindels, DS, Levesque, V, Moffatt, JN, Salih, A, Royant, A & Shaner, NC 2020, 'Aequorea's secrets revealed : new fluorescent proteins with unique properties for bioimaging and biosensing', PLoS Biology, vol. 18, no. 11, e3000936. https://doi.org/10.1371/journal.pbio.3000936

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AU - Lambert, Gerard G.

AU - Depernet, Hadrien

AU - Gotthard, Guillaume

AU - Schultz, Darrin T.

AU - Navizet, Isabelle

AU - Lambert, Talley

AU - Adams, Stephen R.

AU - Torreblanca-Zanca, Albertina

AU - Chu, Meihua

AU - Bindels, Daphne S.

AU - Levesque, Vincent

AU - Moffatt, Jennifer Nero

AU - Salih, Anya

AU - Royant, Antoine

AU - Shaner, Nathan C.

N1 - Publisher Copyright: © 2020 Lambert et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2020/11/2

Y1 - 2020/11/2

N2 - Using mRNA sequencing and de novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent from A. victoria green fluorescent protein (avGFP). Among these FPs are the brightest green fluorescent protein (GFP) homolog yet characterized and a reversibly photochromic FP that responds to UV and blue light. Beyond green emitters, Aequorea species express purple- and blue-pigmented chromoproteins (CPs) with absorbances ranging from green to far-red, including 2 that are photoconvertible. X-ray crystallography revealed that Aequorea CPs contain a chemically novel chromophore with an unexpected crosslink to the main polypeptide chain. Because of the unique attributes of several of these newly discovered FPs, we expect that Aequorea will, once again, give rise to an entirely new generation of useful probes for bioimaging and biosensing.

AB - Using mRNA sequencing and de novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent from A. victoria green fluorescent protein (avGFP). Among these FPs are the brightest green fluorescent protein (GFP) homolog yet characterized and a reversibly photochromic FP that responds to UV and blue light. Beyond green emitters, Aequorea species express purple- and blue-pigmented chromoproteins (CPs) with absorbances ranging from green to far-red, including 2 that are photoconvertible. X-ray crystallography revealed that Aequorea CPs contain a chemically novel chromophore with an unexpected crosslink to the main polypeptide chain. Because of the unique attributes of several of these newly discovered FPs, we expect that Aequorea will, once again, give rise to an entirely new generation of useful probes for bioimaging and biosensing.

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Aequorea's secrets revealed : new fluorescent proteins with unique properties for bioimaging and biosensing

Abstract

Bibliographical note

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