An assessment of the retention behaviour of polycyclic aromatic hydrocarbons on reversed phase stationary phases : thermodynamic behaviour on C18 and phenyl-type surfaces

Sindy Kayillo, Gary R. Dennis, Ross A. Shalliker

    Research output: Contribution to journalArticle

    Abstract

    The thermodynamic retention behaviour of a linear series of polycyclic aromatic hydrocarbons (PAHs) was investigated on C18 and selected phenyl-type reversed-phase stationary phases, namely C18, C18 Aqua, Propyl-phenyl and Synergi polar-RP stationary phases, using methanol mobile phases. The Propyl-phenyl stationary phase, despite having the lowest surface coverage, was found to exhibit significantly larger enthalpic interactions to the other Phenyl-type phase (Synergi polar-RP) even though this had a much higher surface coverage. This indicated that stronger interactions between the PAHs and the stationary phase ligands were occurring on the Propyl-phenyl phase. Evaluation of the elution band profile of the PAHs in the aqueous methanol mobile phase revealed fairly symmetrical bands for the C18, C18 Aqua and Synergi polar-RP, but severe peak tailing on the Propyl-phenyl phase. A change in mobile phase from methanol to acetonitrile improved the peak shape of the PAHs on the Propyl-phenyl phase, leading to the assumption that unfavourable π–π interactions were occurring between the electron-rich PAHs and the electron-rich phenyl rings of the Propyl-phenyl phase.
    Original languageEnglish
    Number of pages8
    JournalJournal of Chromatography A
    DOIs
    Publication statusPublished - 2007

    Keywords

    • PAH
    • phenyl stationary phases
    • retention behaviour
    • thermodynamics

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