Androgen Receptor Variant 7 (AR-V7) Transcript Expression in Circulating Tumor Cells

Tanzila Khan; Yafeng Ma; Alison Luk; Francis Young; Paul de Souza; Wei Chua; Bavanthi Balakrishnar; Therese M. Becker

doi:10.1007/978-1-0716-5050-9_7

Androgen Receptor Variant 7 (AR-V7) Transcript Expression in Circulating Tumor Cells

Tanzila Khan
, Yafeng Ma
, Alison Luk
, Francis Young
, Paul de Souza
, Wei Chua
, Bavanthi Balakrishnar
, Therese M. Becker

Ingham Institute of Applied Medical Research
University of New South Wales
Prince of Wales Hospital
The University of Sydney
Liverpool Hospital

Research output: Chapter in Book / Conference Paper › Chapter › peer-review

Abstract

In recent years, there has been increasing interest in developing drugs that increase the precision targeting of molecular alterations in prostate cancer cells. Traditionally, tissue samples are used to screen for and study such alterations, but liquid biopsies emerge as very practical and potentially superior methodologies to longitudinally characterize cancer cell biology and evolution. The expression of androgen receptor variants correlates with the development of castrate resistance prostate cancer (CRPC), and the most studied variant 7 (AR-V7) is associated with CRPC, disease progression, and resistance to certain AR-pathway targeted treatments and is readily detectable in circulating tumor cells (CTCs). Here, we describe a method of AR-V7 detection in CTCs using highly sensitive droplet digital PCR (ddPCR).

Original language	English
Title of host publication	Circulating Tumor Cells: Methods and Protocols
Publisher	Humana Press Inc
Pages	81-89
Number of pages	9
Volume	2999
DOIs	https://doi.org/10.1007/978-1-0716-5050-9_7
Publication status	Published - 2026
Externally published	Yes

Publication series

Name	Methods in molecular biology (Clifton, N.J.)
Publisher	Humana Press
ISSN (Print)	1064-3745

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

androgen receptor (AR)
Androgen receptor variant 7 (AR-V7)
Circulating tumor cell (CTC)
Droplet digital PCR (ddPCR)
Prostate cancer

Access to Document

10.1007/978-1-0716-5050-9_7

Cite this

Khan, T., Ma, Y., Luk, A., Young, F., de Souza, P., Chua, W., Balakrishnar, B., & Becker, T. M. (2026). Androgen Receptor Variant 7 (AR-V7) Transcript Expression in Circulating Tumor Cells. In Circulating Tumor Cells: Methods and Protocols (Vol. 2999, pp. 81-89). (Methods in molecular biology (Clifton, N.J.)). Humana Press Inc. https://doi.org/10.1007/978-1-0716-5050-9_7

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title = "Androgen Receptor Variant 7 (AR-V7) Transcript Expression in Circulating Tumor Cells",

abstract = "In recent years, there has been increasing interest in developing drugs that increase the precision targeting of molecular alterations in prostate cancer cells. Traditionally, tissue samples are used to screen for and study such alterations, but liquid biopsies emerge as very practical and potentially superior methodologies to longitudinally characterize cancer cell biology and evolution. The expression of androgen receptor variants correlates with the development of castrate resistance prostate cancer (CRPC), and the most studied variant 7 (AR-V7) is associated with CRPC, disease progression, and resistance to certain AR-pathway targeted treatments and is readily detectable in circulating tumor cells (CTCs). Here, we describe a method of AR-V7 detection in CTCs using highly sensitive droplet digital PCR (ddPCR).",

keywords = "androgen receptor (AR), Androgen receptor variant 7 (AR-V7), Circulating tumor cell (CTC), Droplet digital PCR (ddPCR), Prostate cancer",

author = "Tanzila Khan and Yafeng Ma and Alison Luk and Francis Young and \{de Souza\}, Paul and Wei Chua and Bavanthi Balakrishnar and Becker, \{Therese M.\}",

year = "2026",

doi = "10.1007/978-1-0716-5050-9\_7",

language = "English",

volume = "2999",

series = "Methods in molecular biology (Clifton, N.J.)",

publisher = "Humana Press Inc",

pages = "81--89",

booktitle = "Circulating Tumor Cells: Methods and Protocols",

}

TY - CHAP

T1 - Androgen Receptor Variant 7 (AR-V7) Transcript Expression in Circulating Tumor Cells

AU - Khan, Tanzila

AU - Ma, Yafeng

AU - Luk, Alison

AU - Young, Francis

AU - de Souza, Paul

AU - Chua, Wei

AU - Balakrishnar, Bavanthi

AU - Becker, Therese M.

PY - 2026

Y1 - 2026

N2 - In recent years, there has been increasing interest in developing drugs that increase the precision targeting of molecular alterations in prostate cancer cells. Traditionally, tissue samples are used to screen for and study such alterations, but liquid biopsies emerge as very practical and potentially superior methodologies to longitudinally characterize cancer cell biology and evolution. The expression of androgen receptor variants correlates with the development of castrate resistance prostate cancer (CRPC), and the most studied variant 7 (AR-V7) is associated with CRPC, disease progression, and resistance to certain AR-pathway targeted treatments and is readily detectable in circulating tumor cells (CTCs). Here, we describe a method of AR-V7 detection in CTCs using highly sensitive droplet digital PCR (ddPCR).

AB - In recent years, there has been increasing interest in developing drugs that increase the precision targeting of molecular alterations in prostate cancer cells. Traditionally, tissue samples are used to screen for and study such alterations, but liquid biopsies emerge as very practical and potentially superior methodologies to longitudinally characterize cancer cell biology and evolution. The expression of androgen receptor variants correlates with the development of castrate resistance prostate cancer (CRPC), and the most studied variant 7 (AR-V7) is associated with CRPC, disease progression, and resistance to certain AR-pathway targeted treatments and is readily detectable in circulating tumor cells (CTCs). Here, we describe a method of AR-V7 detection in CTCs using highly sensitive droplet digital PCR (ddPCR).

KW - androgen receptor (AR)

KW - Androgen receptor variant 7 (AR-V7)

KW - Circulating tumor cell (CTC)

KW - Droplet digital PCR (ddPCR)

KW - Prostate cancer

UR - https://www.scopus.com/pages/publications/105045089215

U2 - 10.1007/978-1-0716-5050-9_7

DO - 10.1007/978-1-0716-5050-9_7

M3 - Chapter

C2 - 42426450

AN - SCOPUS:105045089215

VL - 2999

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 81

EP - 89

BT - Circulating Tumor Cells: Methods and Protocols

PB - Humana Press Inc

ER -

Androgen Receptor Variant 7 (AR-V7) Transcript Expression in Circulating Tumor Cells

Abstract

Publication series

UN SDGs

Keywords

Access to Document

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