TY - JOUR
T1 - Anomalies in evaporative light scattering detection
AU - Shock, D.
AU - Dennis, G. R.
AU - Guiochon, G.
AU - Dasgupta, P. K.
AU - Shalliker, R. A.
PY - 2011
Y1 - 2011
N2 - A two-dimensional (2-D) " heart-cutting" HPLC system was used to fractionate oligostyrenes into the respective diastereoisomers. For samples of known composition, the response of an ultraviolet (UV) absorbance detector followed the anticipated pattern. The response of an evaporative light-scattering (ELSD) detector on the other hand indicated quite different concentrations for the two diastereoisomers, relative to what was anticipated and what was indicated by the UV detector. Whereas approximately the same concentration was indicated by UV, ELSD in some cases indicated no detection of the later eluting isomer. The magnitude of the errors depended on both the molecular weight and the tacticity of the diastereomers. These anomalies appear to be an artifact of power transform functions imbedded within the firmware processor of the ELSD, invisible to the user.
AB - A two-dimensional (2-D) " heart-cutting" HPLC system was used to fractionate oligostyrenes into the respective diastereoisomers. For samples of known composition, the response of an ultraviolet (UV) absorbance detector followed the anticipated pattern. The response of an evaporative light-scattering (ELSD) detector on the other hand indicated quite different concentrations for the two diastereoisomers, relative to what was anticipated and what was indicated by the UV detector. Whereas approximately the same concentration was indicated by UV, ELSD in some cases indicated no detection of the later eluting isomer. The magnitude of the errors depended on both the molecular weight and the tacticity of the diastereomers. These anomalies appear to be an artifact of power transform functions imbedded within the firmware processor of the ELSD, invisible to the user.
UR - http://handle.uws.edu.au:8081/1959.7/553366
U2 - 10.1016/j.aca.2011.06.060
DO - 10.1016/j.aca.2011.06.060
M3 - Article
SN - 0003-2670
VL - 703
SP - 245
EP - 249
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 2
ER -