TY - JOUR
T1 - Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds
AU - Zhang, Lin
AU - Ravipati, Anjaneya S.
AU - Koyyalamudi, Sundar Rao
AU - Jeong, Sang Chul
AU - Reddy, Narsimha
AU - Smith, Paul T.
AU - Bartlett, John
AU - Shanmugam, Kirubakaran
AU - Münch, Gerald
AU - Wu, Ming Jie
PY - 2011
Y1 - 2011
N2 - The antioxidant, anti-inflammatory, and cytotoxic activities of water and ethanol extracts of 14 Chinese medicinal plants were investigated and also their total phenolics and flavonoid contents measured. The antioxidant activity was evaluated in a biological assay using Saccharomyces cerevisiae, whereas the radical scavenging activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolics and flavonoid contents were estimated by Folin-Ciocalteu and aluminum chloride methods, respectively. The anti-inflammatory activities of the plant extracts were determined by measuring the inhibition of production of nitric oxide (NO) and TNF-α in LPS and IFN-γ activated RAW 264.7 macrophages. Their cytotoxic activities against macrophages were determined by Alamar Blue assay. Four plants, namely, Scutellaria baicalensis, Taxillus chinensis, Rheum officinale, and Sophora japonica, showed significant antioxidant activity in both yeast model and also free radical scavenging methods. The ethanol extract of S. japonica showed highest levels of phenolics and flavonoids (91.33 GAE mg/g and 151.86 QE mg/g, respectively). A positive linear correlation between antioxidant activity and the total phenolics and flavonoid contents indicates that these compounds are likely to be the main antioxidants contributing to the observed activities. Five plant extracts (S. baicalensis, T. chinensis, S. japonica, Mahonia fortunei, and Sophora flavescens) exhibited significant anti-inflammatory activity by in vitro inhibition of the production of NO and TNF-α with low IC₅₀ values. These findings suggest that some of the medicinal herbs studied in this paper are good sources of antioxidants.
AB - The antioxidant, anti-inflammatory, and cytotoxic activities of water and ethanol extracts of 14 Chinese medicinal plants were investigated and also their total phenolics and flavonoid contents measured. The antioxidant activity was evaluated in a biological assay using Saccharomyces cerevisiae, whereas the radical scavenging activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolics and flavonoid contents were estimated by Folin-Ciocalteu and aluminum chloride methods, respectively. The anti-inflammatory activities of the plant extracts were determined by measuring the inhibition of production of nitric oxide (NO) and TNF-α in LPS and IFN-γ activated RAW 264.7 macrophages. Their cytotoxic activities against macrophages were determined by Alamar Blue assay. Four plants, namely, Scutellaria baicalensis, Taxillus chinensis, Rheum officinale, and Sophora japonica, showed significant antioxidant activity in both yeast model and also free radical scavenging methods. The ethanol extract of S. japonica showed highest levels of phenolics and flavonoids (91.33 GAE mg/g and 151.86 QE mg/g, respectively). A positive linear correlation between antioxidant activity and the total phenolics and flavonoid contents indicates that these compounds are likely to be the main antioxidants contributing to the observed activities. Five plant extracts (S. baicalensis, T. chinensis, S. japonica, Mahonia fortunei, and Sophora flavescens) exhibited significant anti-inflammatory activity by in vitro inhibition of the production of NO and TNF-α with low IC₅₀ values. These findings suggest that some of the medicinal herbs studied in this paper are good sources of antioxidants.
KW - aluminum chloride
KW - anti-inflammatory agents
KW - antineoplastic agents
KW - antioxidants
KW - flavonoids
KW - macrophages
KW - medicinal plants
KW - medicine, Chinese
KW - phenols
UR - http://handle.uws.edu.au:8081/1959.7/526382
U2 - 10.1021/jf203146e
DO - 10.1021/jf203146e
M3 - Article
SN - 0021-8561
VL - 59
SP - 12361
EP - 12367
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 23
ER -