Apigenin impedes cell cycle progression at G2 phase in prostate cancer cells

Su Su Thae Hnit, Mu Yao, Chanlu Xie, Ling Bi, Matthew Wong, Tao Liu, Paul De Souza, Zhong Li, Qihan Dong

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

As a natural flavone, apigenin is abundantly present in vegetables, fruits, oregano, tea, chamomile, wheat sprout and is regarded as a major component of the Mediterranean diet. Apigenin is known to inhibit proliferation in different cancer cell lines by inducing G2/M arrest, but it is unclear whether this action is predominantly imposed on G2 or M phases. In this study, we demonstrate that apigenin arrests prostate cancer cells at G2 phase by flow cytometric analysis of prostate cancer cells co-stained for phospho-Histone H3 and DNA. Concurrently, apigenin also reduces the mRNA and protein levels of the key regulators that govern G2-M transition. Further analysis using chromatin immunoprecipitation (ChIP) confirmed the diminished transcriptional activities of the genes coding for these regulators. Unravelling the inhibitory effect of apigenin on G2-M transition in cancer cells provides the mechanistic understanding of its action and supports the potential for apigenin as an anti-cancer agent.
Original languageEnglish
Article number44
Number of pages12
JournalDiscover Oncology
Volume13
Issue number1
DOIs
Publication statusPublished - 2022

Open Access - Access Right Statement

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Fingerprint

Dive into the research topics of 'Apigenin impedes cell cycle progression at G2 phase in prostate cancer cells'. Together they form a unique fingerprint.

Cite this