Application of fingerprinting molecular methods in bioremediation studies

Dimitrios G. Karpouzas, Brajesh K. Singh

    Research output: Chapter in Book / Conference PaperChapter

    7 Citations (Scopus)

    Abstract

    Bioremediation has been identified as a beneficial and effective strategy for the removal of recalcitrant environmental contaminants. Bioaugmentation of polluted environments with exogenous degrading microorganisms constitutes a major strategy of bioremediation. However, the ecological role of these strains and their impact on the endogenous microbial community of the micro-ecosystems where they are released should be known. Fingerprinting PCR-based methods, like denaturating gradient gel electrophoresis(DGGE) and terminal restriction fragment length polymorphism (TRFLP), could be used in studies exploring the ecology of pollutant-degrading microorganisms and their effects on the structure of the soil microbial community. This chapter provides a brief outline of the technical details involved in the application of DGGE and TRFLP fingerprinting in soil microbial ecology, with particular reference to bioremediation studies.
    Original languageEnglish
    Title of host publicationBioremediation: Methods and Protocols
    EditorsStephen P. Cummings
    Place of PublicationU.S.
    PublisherHuman Press
    Pages69-88
    Number of pages20
    ISBN (Electronic)9781607614395
    ISBN (Print)9781607614388
    DOIs
    Publication statusPublished - 2010

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