Abstract
Bioremediation has been identified as a beneficial and effective strategy for the removal of recalcitrant environmental contaminants. Bioaugmentation of polluted environments with exogenous degrading microorganisms constitutes a major strategy of bioremediation. However, the ecological role of these strains and their impact on the endogenous microbial community of the micro-ecosystems where they are released should be known. Fingerprinting PCR-based methods, like denaturating gradient gel electrophoresis(DGGE) and terminal restriction fragment length polymorphism (TRFLP), could be used in studies exploring the ecology of pollutant-degrading microorganisms and their effects on the structure of the soil microbial community. This chapter provides a brief outline of the technical details involved in the application of DGGE and TRFLP fingerprinting in soil microbial ecology, with particular reference to bioremediation studies.
Original language | English |
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Title of host publication | Bioremediation: Methods and Protocols |
Editors | Stephen P. Cummings |
Place of Publication | U.S. |
Publisher | Human Press |
Pages | 69-88 |
Number of pages | 20 |
ISBN (Electronic) | 9781607614395 |
ISBN (Print) | 9781607614388 |
DOIs | |
Publication status | Published - 2010 |