Applications of fluorescence spectroscopy and confocal microscopy

Anya Salih, Paul Wormell, K. Benjamin Garbutcheon-Singh, Benjamin Harper, Simon Myers, David Geny, Christopher Hammang, Janice Aldrich-Wright

    Research output: Chapter in Book / Conference PaperChapter

    Abstract

    In this chapter we describe the use of fluorescence spectroscopy and confocal microscopy to investigate the DNA binding capacity, uptake route and intracellular localisation of novel metallointercalators. Fluorescence is the property whereby some atoms or molecules absorb UV or visible light and then re-emit it at longer wavelengths after a brief interval, termed the fluorescence lifetime.
    Original languageEnglish
    Title of host publicationMetallointercalators: Synthesis and Techniques to Probe Their Interactions with Biomolecules
    EditorsJanice R. Aldrich-Wright
    Place of PublicationU.S.
    PublisherSpringer
    Pages235-272
    Number of pages38
    ISBN (Electronic)9783211990797
    ISBN (Print)9783211094273
    DOIs
    Publication statusPublished - 2011

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