Aptamers : molecular binding agents with application in targeted cancer treatment

Nikita Orkey

Aptamers : molecular binding agents with application in targeted cancer treatment

Nikita Orkey

Research output: Contribution to journal › Article

Abstract

Aptamers are single stranded (ss) DNA or RNA oligonucleotides consisting of 25 to more than 200 nucleosides that can fold into three-dimensional structures and bind selectively to antigens on the surface of human cells, as well as small chemicals and proteins. Aptamers are selected from random sequence libraries and synthesized using a completely automated process called systematic evolution of ligands by exponential enrichment or SELEX (Fig. 1). They can also be chemically synthesized using solid-phase phosphoramidite chemistry. Alteration of their sugar residues from 2ÃƒÂ¢Ã¢"šÂ¬Ã‚Â²-hydroxyl group of the ribose to 2ÃƒÂ¢Ã¢"šÂ¬Ã‚Â²-fluoro or 2ÃƒÂ¢Ã¢"šÂ¬Ã‚Â²-amino make aptamers resistant to cleavage by cellular enzymes. Poly(ethyleneglycol) (PEGylation) can be used to increase clearance times of the aptamer from blood serum.

Original language	English
Number of pages	1
Journal	Australian Journal of Chemistry
Publication status	Published - 2007

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

aptamers
cancer treatment
molecular binding agents
systematic evolution of ligands

Cite this

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title = "Aptamers : molecular binding agents with application in targeted cancer treatment",

abstract = "Aptamers are single stranded (ss) DNA or RNA oligonucleotides consisting of 25 to more than 200 nucleosides that can fold into three-dimensional structures and bind selectively to antigens on the surface of human cells, as well as small chemicals and proteins. Aptamers are selected from random sequence libraries and synthesized using a completely automated process called systematic evolution of ligands by exponential enrichment or SELEX (Fig. 1). They can also be chemically synthesized using solid-phase phosphoramidite chemistry. Alteration of their sugar residues from 2{\~A}ƒ{\^A}¢{\~A}¢{"}{\v s}{\^A}¬{\~A}‚{\^A}²-hydroxyl group of the ribose to 2{\~A}ƒ{\^A}¢{\~A}¢{"}{\v s}{\^A}¬{\~A}‚{\^A}²-fluoro or 2{\~A}ƒ{\^A}¢{\~A}¢{"}{\v s}{\^A}¬{\~A}‚{\^A}²-amino make aptamers resistant to cleavage by cellular enzymes. Poly(ethyleneglycol) (PEGylation) can be used to increase clearance times of the aptamer from blood serum.",

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author = "Nikita Orkey",

year = "2007",

language = "English",

journal = "Australian Journal of Chemistry",

publisher = "CSIRO",

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TY - JOUR

T1 - Aptamers : molecular binding agents with application in targeted cancer treatment

AU - Orkey, Nikita

PY - 2007

Y1 - 2007

N2 - Aptamers are single stranded (ss) DNA or RNA oligonucleotides consisting of 25 to more than 200 nucleosides that can fold into three-dimensional structures and bind selectively to antigens on the surface of human cells, as well as small chemicals and proteins. Aptamers are selected from random sequence libraries and synthesized using a completely automated process called systematic evolution of ligands by exponential enrichment or SELEX (Fig. 1). They can also be chemically synthesized using solid-phase phosphoramidite chemistry. Alteration of their sugar residues from 2ÃƒÂ¢Ã¢"šÂ¬Ã‚Â²-hydroxyl group of the ribose to 2ÃƒÂ¢Ã¢"šÂ¬Ã‚Â²-fluoro or 2ÃƒÂ¢Ã¢"šÂ¬Ã‚Â²-amino make aptamers resistant to cleavage by cellular enzymes. Poly(ethyleneglycol) (PEGylation) can be used to increase clearance times of the aptamer from blood serum.

AB - Aptamers are single stranded (ss) DNA or RNA oligonucleotides consisting of 25 to more than 200 nucleosides that can fold into three-dimensional structures and bind selectively to antigens on the surface of human cells, as well as small chemicals and proteins. Aptamers are selected from random sequence libraries and synthesized using a completely automated process called systematic evolution of ligands by exponential enrichment or SELEX (Fig. 1). They can also be chemically synthesized using solid-phase phosphoramidite chemistry. Alteration of their sugar residues from 2ÃƒÂ¢Ã¢"šÂ¬Ã‚Â²-hydroxyl group of the ribose to 2ÃƒÂ¢Ã¢"šÂ¬Ã‚Â²-fluoro or 2ÃƒÂ¢Ã¢"šÂ¬Ã‚Â²-amino make aptamers resistant to cleavage by cellular enzymes. Poly(ethyleneglycol) (PEGylation) can be used to increase clearance times of the aptamer from blood serum.

KW - aptamers

KW - cancer treatment

KW - molecular binding agents

KW - systematic evolution of ligands

UR - http://handle.uws.edu.au:8081/1959.7/33994

M3 - Article

JO - Australian Journal of Chemistry

JF - Australian Journal of Chemistry

ER -

Aptamers : molecular binding agents with application in targeted cancer treatment

Abstract

UN SDGs

Keywords

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