Assessment of human colorectal biopsies by ¹H MRS: Correlation with histopathology

Samples (3 mm³ of histopathologically normal (n = 15) and carclnomatous tissue (n = 15) were obtained from colectomy specimens and examined by ¹H MRS. A combination of one‐and two‐dimensional spectra, obtained with appropriate acquisition and processing parameters, provide multiple diagnostic parameters allowing the distinction between normal and carcinomatous tissue. The diagnostic parameters include rosonances from choline, choline‐based, and other metabolites, cell surface fucosylation, and altered lipid profiles. Tissues histopathologically classified as normal, while remaining distinct from the malignant spectral profile, were found to fit into two categories, one of which had some of the spectral characteristics of malignancy. These results indicate that ¹H MRS identifies abnormal colorectal mucosa, which is not morphologically manifest.Such abnormalities have been reported previously to exist in premalignant colorectal tissue by monoclonal antibody studies. Collectively, these results suggest that a clinical study of colorectal biopsies by ¹H MRS could provide support for the use of MRS as an adjunct to current pathological procedures.