Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1

Soumya Joseph; Ann H. Y. Kwan; Joel P. Mackay; Liza Cubeddu; Jacqueline M. Matthews

doi:10.1007/s12104-013-9470-x

Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1

Soumya Joseph, Ann H. Y. Kwan, Joel P. Mackay, Liza Cubeddu, Jacqueline M. Matthews

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The transcriptional regulator LMO4 and the transcription factor DEAF-1 are both essential for brain and skeletal development. They are also implicated in human breast cancers; overexpression of LMO4 is an indicator of poor prognosis, and overexpression of DEAF-1 promotes epithelial breast cell proliferation. We have generated a stable LMO4–DEAF-1 complex comprising the C-terminal LIM domain of LMO4 and an intrinsically disordered LMO4-interaction domain from DEAF-1 tethered by a glycine/serine linker. Here we report the 1H, 15N and 13C assignments of this construct. Analysis of the assignments indicates the presence of structure in the DEAF-1 part of the complex supporting the presence of a physical interaction between the two proteins.

Original language	English
Pages (from-to)	141-144
Number of pages	4
Journal	Biomolecular NMR Assignments
Volume	8
Issue number	1
DOIs	https://doi.org/10.1007/s12104-013-9470-x
Publication status	Published - 2014

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abstract = "The transcriptional regulator LMO4 and the transcription factor DEAF-1 are both essential for brain and skeletal development. They are also implicated in human breast cancers; overexpression of LMO4 is an indicator of poor prognosis, and overexpression of DEAF-1 promotes epithelial breast cell proliferation. We have generated a stable LMO4–DEAF-1 complex comprising the C-terminal LIM domain of LMO4 and an intrinsically disordered LMO4-interaction domain from DEAF-1 tethered by a glycine/serine linker. Here we report the 1H, 15N and 13C assignments of this construct. Analysis of the assignments indicates the presence of structure in the DEAF-1 part of the complex supporting the presence of a physical interaction between the two proteins.",

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AU - Joseph, Soumya

AU - Kwan, Ann H. Y.

AU - Mackay, Joel P.

AU - Cubeddu, Liza

AU - Matthews, Jacqueline M.

PY - 2014

Y1 - 2014

N2 - The transcriptional regulator LMO4 and the transcription factor DEAF-1 are both essential for brain and skeletal development. They are also implicated in human breast cancers; overexpression of LMO4 is an indicator of poor prognosis, and overexpression of DEAF-1 promotes epithelial breast cell proliferation. We have generated a stable LMO4–DEAF-1 complex comprising the C-terminal LIM domain of LMO4 and an intrinsically disordered LMO4-interaction domain from DEAF-1 tethered by a glycine/serine linker. Here we report the 1H, 15N and 13C assignments of this construct. Analysis of the assignments indicates the presence of structure in the DEAF-1 part of the complex supporting the presence of a physical interaction between the two proteins.

AB - The transcriptional regulator LMO4 and the transcription factor DEAF-1 are both essential for brain and skeletal development. They are also implicated in human breast cancers; overexpression of LMO4 is an indicator of poor prognosis, and overexpression of DEAF-1 promotes epithelial breast cell proliferation. We have generated a stable LMO4–DEAF-1 complex comprising the C-terminal LIM domain of LMO4 and an intrinsically disordered LMO4-interaction domain from DEAF-1 tethered by a glycine/serine linker. Here we report the 1H, 15N and 13C assignments of this construct. Analysis of the assignments indicates the presence of structure in the DEAF-1 part of the complex supporting the presence of a physical interaction between the two proteins.

UR - http://handle.uws.edu.au:8081/1959.7/538299

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DO - 10.1007/s12104-013-9470-x

M3 - Article

SN - 1874-270X

VL - 8

SP - 141

EP - 144

JO - Biomolecular NMR Assignments

JF - Biomolecular NMR Assignments

IS - 1

ER -

Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1

Abstract

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