Better than bench top : high speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography

Thirada Suktham, Andrew Jones, Agustín Acquaviva, Gary R. Dennis, R. Andrew Shalliker, Arianne Soliven

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the main improvements to transform the quantitative protocol into a high-speed qualitative automated assay to screen samples for their potential total antioxidant capacity, typically performed via manual mixing of the sample and derivatisation and measured on a 96 well plate reader/bench top UV–Vis spectrophotometer. This approach with automated mixing is a more informative alternative for total antioxidant capacity as the antioxidant peaks are profiled for each sample within four minutes. This antioxidant profile may be used for routine analysis of raw materials and/or a guide for targeted approaches for structure elucidation for laboratories interested in early drug discovery, natural product research and the search of alternative antioxidant additives in consumer goods/therapeutics. This technique could also be used to monitor the stability, alteration or adulteration of manufactured goods containing antioxidants.
Original languageEnglish
Article number104348
Number of pages6
JournalMicrochemical Journal
Volume152
DOIs
Publication statusPublished - 2020

Keywords

  • antioxidants
  • chromatographic analysis
  • high performance liquid chromatography

Fingerprint

Dive into the research topics of 'Better than bench top : high speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography'. Together they form a unique fingerprint.

Cite this