Biomarkers for severity of spinal cord injury in the cerebrospinal fluid of rats

Joanna M. Lubieniecka; Femke Streijger; Jae H. T. Lee; Nikolay Stoynov; Jie Liu; Randy Mottus; Tom Pfeifer; Brian K. Kwon; Jens R. Coorssen; Leonard J. Foster; Thomas A. Grigliatti; Wolfram Tetzlaff

doi:10.1371/journal.pone.0019247

Biomarkers for severity of spinal cord injury in the cerebrospinal fluid of rats

Joanna M. Lubieniecka, Femke Streijger, Jae H. T. Lee, Nikolay Stoynov, Jie Liu, Randy Mottus, Tom Pfeifer, Brian K. Kwon, Jens R. Coorssen, Leonard J. Foster, Thomas A. Grigliatti, Wolfram Tetzlaff

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60 Citations (Scopus)

Abstract

One of the major challenges in management of spinal cord injury (SCI) is that the assessment of injury severity is often imprecise. Identification of reliable, easily quantifiable biomarkers that delineate the severity of the initial injury and that have prognostic value for the degree of functional recovery would significantly aid the clinician in the choice of potential treatments. To find such biomarkers we performed quantitative liquid chromatography-mass spectrometry (LC-MS/MS) analyses of cerebrospinal fluid (CSF) collected from rats 24 h after either a moderate or severe SCI. We identified a panel of 42 putative biomarkers of SCI, 10 of which represent potential biomarkers of SCI severity. Three of the candidate biomarkers, Ywhaz, Itih4, and Gpx3 were also validated by Western blot in a biological replicate of the injury. The putative biomarkers identified in this study may potentially be a valuable tool in the assessment of the extent of spinal cord damage.

Original language	English
Article number	e19247
Number of pages	13
Journal	PLoS One
Volume	6
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0019247
Publication status	Published - 2011

Open Access - Access Right Statement

Copyright: 2011 Lubieniecka et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Keywords

Western immunoblotting
biochemical markers
cerebrospinal fluid
glutathione
liquid chromatography
mass spectrometry
nerve tissue proteins
rats
spinal cord
trypsin inhibitors
wounds and injuries

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10.1371/journal.pone.0019247

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title = "Biomarkers for severity of spinal cord injury in the cerebrospinal fluid of rats",

abstract = "One of the major challenges in management of spinal cord injury (SCI) is that the assessment of injury severity is often imprecise. Identification of reliable, easily quantifiable biomarkers that delineate the severity of the initial injury and that have prognostic value for the degree of functional recovery would significantly aid the clinician in the choice of potential treatments. To find such biomarkers we performed quantitative liquid chromatography-mass spectrometry (LC-MS/MS) analyses of cerebrospinal fluid (CSF) collected from rats 24 h after either a moderate or severe SCI. We identified a panel of 42 putative biomarkers of SCI, 10 of which represent potential biomarkers of SCI severity. Three of the candidate biomarkers, Ywhaz, Itih4, and Gpx3 were also validated by Western blot in a biological replicate of the injury. The putative biomarkers identified in this study may potentially be a valuable tool in the assessment of the extent of spinal cord damage.",

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author = "Lubieniecka, {Joanna M.} and Femke Streijger and Lee, {Jae H. T.} and Nikolay Stoynov and Jie Liu and Randy Mottus and Tom Pfeifer and Kwon, {Brian K.} and Coorssen, {Jens R.} and Foster, {Leonard J.} and Grigliatti, {Thomas A.} and Wolfram Tetzlaff",

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AU - Lubieniecka, Joanna M.

AU - Streijger, Femke

AU - Lee, Jae H. T.

AU - Stoynov, Nikolay

AU - Liu, Jie

AU - Mottus, Randy

AU - Pfeifer, Tom

AU - Kwon, Brian K.

AU - Coorssen, Jens R.

AU - Foster, Leonard J.

AU - Grigliatti, Thomas A.

AU - Tetzlaff, Wolfram

PY - 2011

Y1 - 2011

N2 - One of the major challenges in management of spinal cord injury (SCI) is that the assessment of injury severity is often imprecise. Identification of reliable, easily quantifiable biomarkers that delineate the severity of the initial injury and that have prognostic value for the degree of functional recovery would significantly aid the clinician in the choice of potential treatments. To find such biomarkers we performed quantitative liquid chromatography-mass spectrometry (LC-MS/MS) analyses of cerebrospinal fluid (CSF) collected from rats 24 h after either a moderate or severe SCI. We identified a panel of 42 putative biomarkers of SCI, 10 of which represent potential biomarkers of SCI severity. Three of the candidate biomarkers, Ywhaz, Itih4, and Gpx3 were also validated by Western blot in a biological replicate of the injury. The putative biomarkers identified in this study may potentially be a valuable tool in the assessment of the extent of spinal cord damage.

AB - One of the major challenges in management of spinal cord injury (SCI) is that the assessment of injury severity is often imprecise. Identification of reliable, easily quantifiable biomarkers that delineate the severity of the initial injury and that have prognostic value for the degree of functional recovery would significantly aid the clinician in the choice of potential treatments. To find such biomarkers we performed quantitative liquid chromatography-mass spectrometry (LC-MS/MS) analyses of cerebrospinal fluid (CSF) collected from rats 24 h after either a moderate or severe SCI. We identified a panel of 42 putative biomarkers of SCI, 10 of which represent potential biomarkers of SCI severity. Three of the candidate biomarkers, Ywhaz, Itih4, and Gpx3 were also validated by Western blot in a biological replicate of the injury. The putative biomarkers identified in this study may potentially be a valuable tool in the assessment of the extent of spinal cord damage.

KW - Western immunoblotting

KW - biochemical markers

KW - cerebrospinal fluid

KW - glutathione

KW - liquid chromatography

KW - mass spectrometry

KW - nerve tissue proteins

KW - rats

KW - spinal cord

KW - trypsin inhibitors

KW - wounds and injuries

UR - http://hdl.handle.net/1959.7/uws:15803

U2 - 10.1371/journal.pone.0019247

DO - 10.1371/journal.pone.0019247

M3 - Article

SN - 1932-6203

VL - 6

JO - PLoS One

JF - PLoS One

IS - 4

M1 - e19247

ER -

Biomarkers for severity of spinal cord injury in the cerebrospinal fluid of rats

Abstract

Open Access - Access Right Statement

Keywords

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