Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology

Paul P. Breen; Yossi Buskila

doi:10.1109/EMBC.2014.6944713

Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology

Research output: Chapter in Book / Conference Paper › Conference Paper › peer-review

14 Citations (Scopus)

Abstract

In vitro brain slice preparations are instrumental in developing our understanding of the nervous system. However, the current lifespan of an acute brain slice is limited to approximately 6-12 hours. This reduces potential experimentation time and leads to considerable waste of neural tissue. We have designed, developed and tested a novel incubation system capable of extending the lifespan of these brain slices. This is done by controlling the temperature and pH of the artificial cerebral spinal fluid in which the slices are incubated while continuously passing the fluid through a UVC filtration system. This system is capable of maintaining extremely low bacterial levels and significantly extending the brain slice lifespan to at least 24 hours. Brain slice viability was validated through electrophysiological recordings as well as live/dead cell assays.

Original language	English
Title of host publication	Proceedings of 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2014), Chicago, Illinois, USA, 26-30 August 2014
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	4864-4867
Number of pages	4
ISBN (Electronic)	9781424479290
ISBN (Print)	9781424479276
DOIs	https://doi.org/10.1109/EMBC.2014.6944713
Publication status	Published - 2014
Event	IEEE Engineering in Medicine and Biology Society. Annual Conference - Duration: 30 Apr 2015 → …

Publication series

Name	2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014

Conference

Conference	IEEE Engineering in Medicine and Biology Society. Annual Conference
Period	30/04/15 → …

Bibliographical note

Publisher Copyright:
© 2014 IEEE.

Keywords

brain
in vitro
incubators
microorganisms
neurophysiology

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10.1109/EMBC.2014.6944713

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Breen, P. P., & Buskila, Y. (2014). Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology. In Proceedings of 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2014), Chicago, Illinois, USA, 26-30 August 2014 (pp. 4864-4867). Article 6944713 (2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2014.6944713

Breen, Paul P. ; Buskila, Yossi. / Braincubator : An incubation system to extend brain slice lifespan for use in neurophysiology. Proceedings of 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2014), Chicago, Illinois, USA, 26-30 August 2014. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 4864-4867 (2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014).

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abstract = "In vitro brain slice preparations are instrumental in developing our understanding of the nervous system. However, the current lifespan of an acute brain slice is limited to approximately 6-12 hours. This reduces potential experimentation time and leads to considerable waste of neural tissue. We have designed, developed and tested a novel incubation system capable of extending the lifespan of these brain slices. This is done by controlling the temperature and pH of the artificial cerebral spinal fluid in which the slices are incubated while continuously passing the fluid through a UVC filtration system. This system is capable of maintaining extremely low bacterial levels and significantly extending the brain slice lifespan to at least 24 hours. Brain slice viability was validated through electrophysiological recordings as well as live/dead cell assays.",

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doi = "10.1109/EMBC.2014.6944713",

language = "English",

isbn = "9781424479276",

series = "2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

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booktitle = "Proceedings of 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2014), Chicago, Illinois, USA, 26-30 August 2014",

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Breen, PP & Buskila, Y 2014, Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology. in Proceedings of 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2014), Chicago, Illinois, USA, 26-30 August 2014., 6944713, 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014, Institute of Electrical and Electronics Engineers Inc., pp. 4864-4867, IEEE Engineering in Medicine and Biology Society. Annual Conference, 30/04/15. https://doi.org/10.1109/EMBC.2014.6944713

Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology. / Breen, Paul P.; Buskila, Yossi.
Proceedings of 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2014), Chicago, Illinois, USA, 26-30 August 2014. Institute of Electrical and Electronics Engineers Inc., 2014. p. 4864-4867 6944713 (2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014).

Research output: Chapter in Book / Conference Paper › Conference Paper › peer-review

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Breen PP , Buskila Y. Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology. In Proceedings of 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2014), Chicago, Illinois, USA, 26-30 August 2014. Institute of Electrical and Electronics Engineers Inc. 2014. p. 4864-4867. 6944713. (2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014). doi: 10.1109/EMBC.2014.6944713

Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology

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