Ca2+ sensors of L-type Ca2+ channel

Christoph Romanin; Roland Gamsjaeger; Heike Kahr; Daniela Schaufler; Olga Carlson; Darrell R. Abernethy; Nikolai M. Soldatov

doi:10.1016/S0014-5793(00)02361-9

Ca2+ sensors of L-type Ca2+ channel

Christoph Romanin, Roland Gamsjaeger, Heike Kahr, Daniela Schaufler, Olga Carlson, Darrell R. Abernethy, Nikolai M. Soldatov

Western Sydney University

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

Ca2+-induced inactivation of L-type Ca2+ is differentially mediated by two C-terminal motifs of the α1C subunit, L (1572–1587) and K (1599–1651) implicated for calmodulin binding. We found that motif L is composed of a highly selective Ca2+ sensor and an adjacent Ca2+-independent tethering site for calmodulin. The Ca2+ sensor contributes to higher Ca2+ sensitivity of the motif L complex with calmodulin. Since only combined mutation of both sites removes Ca2+-dependent current decay, the two-site modulation by Ca2+ and calmodulin may underlie Ca2+-induced inactivation of the channel.

Original language	English
Pages (from-to)	301-306
Number of pages	6
Journal	FEBS Letters
Volume	487
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(00)02361-9
Publication status	Published - 2000

Keywords

calcium channels
calmodulin
heart
Heart
Inactivation
Calcium channel
Ca sensor
Calmodulin

Access to Document

10.1016/S0014-5793(00)02361-9

Cite this

@article{1fda6dd3ae9d492db07de13b31903653,

title = "Ca2+ sensors of L-type Ca2+ channel",

abstract = "Ca2+-induced inactivation of L-type Ca2+ is differentially mediated by two C-terminal motifs of the α1C subunit, L (1572–1587) and K (1599–1651) implicated for calmodulin binding. We found that motif L is composed of a highly selective Ca2+ sensor and an adjacent Ca2+-independent tethering site for calmodulin. The Ca2+ sensor contributes to higher Ca2+ sensitivity of the motif L complex with calmodulin. Since only combined mutation of both sites removes Ca2+-dependent current decay, the two-site modulation by Ca2+ and calmodulin may underlie Ca2+-induced inactivation of the channel.",

keywords = "calcium channels, calmodulin, heart, Heart, Inactivation, Calcium channel, Ca sensor, Calmodulin",

author = "Christoph Romanin and Roland Gamsjaeger and Heike Kahr and Daniela Schaufler and Olga Carlson and Abernethy, {Darrell R.} and Soldatov, {Nikolai M.}",

year = "2000",

doi = "10.1016/S0014-5793(00)02361-9",

language = "English",

volume = "487",

pages = "301--306",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley & Sons",

number = "2",

}

TY - JOUR

T1 - Ca2+ sensors of L-type Ca2+ channel

AU - Romanin, Christoph

AU - Gamsjaeger, Roland

AU - Kahr, Heike

AU - Schaufler, Daniela

AU - Carlson, Olga

AU - Abernethy, Darrell R.

AU - Soldatov, Nikolai M.

PY - 2000

Y1 - 2000

N2 - Ca2+-induced inactivation of L-type Ca2+ is differentially mediated by two C-terminal motifs of the α1C subunit, L (1572–1587) and K (1599–1651) implicated for calmodulin binding. We found that motif L is composed of a highly selective Ca2+ sensor and an adjacent Ca2+-independent tethering site for calmodulin. The Ca2+ sensor contributes to higher Ca2+ sensitivity of the motif L complex with calmodulin. Since only combined mutation of both sites removes Ca2+-dependent current decay, the two-site modulation by Ca2+ and calmodulin may underlie Ca2+-induced inactivation of the channel.

AB - Ca2+-induced inactivation of L-type Ca2+ is differentially mediated by two C-terminal motifs of the α1C subunit, L (1572–1587) and K (1599–1651) implicated for calmodulin binding. We found that motif L is composed of a highly selective Ca2+ sensor and an adjacent Ca2+-independent tethering site for calmodulin. The Ca2+ sensor contributes to higher Ca2+ sensitivity of the motif L complex with calmodulin. Since only combined mutation of both sites removes Ca2+-dependent current decay, the two-site modulation by Ca2+ and calmodulin may underlie Ca2+-induced inactivation of the channel.

KW - calcium channels

KW - calmodulin

KW - heart

KW - Heart

KW - Inactivation

KW - Calcium channel

KW - Ca sensor

KW - Calmodulin

UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:44679

UR - http://www.scopus.com/inward/record.url?scp=0034731486&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(00)02361-9

DO - 10.1016/S0014-5793(00)02361-9

M3 - Article

SN - 0014-5793

VL - 487

SP - 301

EP - 306

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Ca2+ sensors of L-type Ca2+ channel

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this