Calcium imaging of retinal ganglion cell dendritic responses to extracellular microelectrode stimulation

Chih-Yu Yang, Andrew J. Woolley, David Tsai, Gregg J. Suaning, John W. Morley, Nigel H. Lovell

    Research output: Chapter in Book / Conference PaperConference Paperpeer-review

    1 Citation (Scopus)

    Abstract

    ![CDATA[In order to improve upon existing stimulation strategies for prosthetic vision, it is essential to assess stimulation effectiveness by measuring the responses of stimulated cells in retinal tissue. Calcium imaging has slower dynamics and lower signal-to-noise ratio than electrophysiological techniques. However, it has the ability to record responses from a large number of neurons, and is able to monitor responses from neurites too small for patch electrodes. We extended previous techniques of bulk loading RGC somata with organic calcium indicator to achieve staining of RGC dendritic processes and imaged calcium signals in retinal ganglion cell somata and dendrites simultaneously. The higher signal-to-noise ratio dendritic calcium signals make dendrites more suitable imaging regions for recording retinal ganglion cell responses to electrical stimulation protocols that are relevant to driving visual prosthetic stimulation.]]
    Original languageEnglish
    Title of host publicationProceedings of the 7th Annual International IEEE EMBS Conference on Neural Engineering, Montpellier, France, 22-24 April 2015
    PublisherIEEE
    Pages438-441
    Number of pages4
    ISBN (Print)9781467363891
    DOIs
    Publication statusPublished - 2015
    EventInternational IEEE EMBS Conference on Neural Engineering -
    Duration: 22 Apr 2015 → …

    Publication series

    Name
    ISSN (Print)1948-3546

    Conference

    ConferenceInternational IEEE EMBS Conference on Neural Engineering
    Period22/04/15 → …

    Keywords

    • calcium
    • microelectrodes
    • ophthalmology
    • retinal ganglion cells

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