Cellular response to linear and branched poly(acrylic acid)a

Elizabeth G. Whitty; Alison R. Maniego; Sharon A. Bentwitch; Yohann Guillaneuf; Mark R. Jones; Marianne Gaborieau; Patrice Castignolles

doi:10.1002/mabi.201500153

Cellular response to linear and branched poly(acrylic acid)a

Elizabeth G. Whitty, Alison R. Maniego, Sharon A. Bentwitch, Yohann Guillaneuf, Mark R. Jones, Marianne Gaborieau, Patrice Castignolles

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Poly(acrylic acid-co-sodium acrylate) (PNaA) is a pH-responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3-arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72h MTT assay most PNaAs exhibited a IC<inf>50</inf> between 7 and 14mmolL-1, showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3-arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.

Original language	English
Pages (from-to)	1724-1734
Number of pages	11
Journal	Macromolecular Bioscience
Volume	15
Issue number	12
DOIs	https://doi.org/10.1002/mabi.201500153
Publication status	Published - 2015

Keywords

acrylic acid
body fluids
capillary electrophoresis
cells
cytology
organic acids

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/mabi.201500153

Cite this

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title = "Cellular response to linear and branched poly(acrylic acid)a",

abstract = "Poly(acrylic acid-co-sodium acrylate) (PNaA) is a pH-responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3-arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72h MTT assay most PNaAs exhibited a IC50 between 7 and 14mmolL-1, showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3-arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.",

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author = "Whitty, {Elizabeth G.} and Maniego, {Alison R.} and Bentwitch, {Sharon A.} and Yohann Guillaneuf and Jones, {Mark R.} and Marianne Gaborieau and Patrice Castignolles",

year = "2015",

doi = "10.1002/mabi.201500153",

language = "English",

volume = "15",

pages = "1724--1734",

journal = "Macromolecular Bioscience",

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publisher = "Wiley-VCH Verlag GmbH & Co KGaA",

number = "12",

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TY - JOUR

T1 - Cellular response to linear and branched poly(acrylic acid)a

AU - Whitty, Elizabeth G.

AU - Maniego, Alison R.

AU - Bentwitch, Sharon A.

AU - Guillaneuf, Yohann

AU - Jones, Mark R.

AU - Gaborieau, Marianne

AU - Castignolles, Patrice

PY - 2015

Y1 - 2015

N2 - Poly(acrylic acid-co-sodium acrylate) (PNaA) is a pH-responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3-arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72h MTT assay most PNaAs exhibited a IC50 between 7 and 14mmolL-1, showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3-arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.

AB - Poly(acrylic acid-co-sodium acrylate) (PNaA) is a pH-responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3-arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72h MTT assay most PNaAs exhibited a IC50 between 7 and 14mmolL-1, showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3-arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.

KW - acrylic acid

KW - body fluids

KW - capillary electrophoresis

KW - cells

KW - cytology

KW - organic acids

UR - http://handle.uws.edu.au:8081/1959.7/uws:31734

U2 - 10.1002/mabi.201500153

DO - 10.1002/mabi.201500153

M3 - Article

SN - 1616-5187

VL - 15

SP - 1724

EP - 1734

JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

IS - 12

ER -

Cellular response to linear and branched poly(acrylic acid)a

Abstract

Keywords

UN SDGs

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