@inproceedings{3ee2bb70156945b598f45570d6354201,
title = "Centrifugal microfluidic sample lysis and homogenization platform for nucleic acid extraction",
abstract = "The application of microfluidic technologies for nucleic acid diagnostics continues to expand. While attention has primarily focused on nucleic acid amplification and detection, little consideration has been paid to sample preparation. Here, a centrifugal microfluidic platform for mechanical sample lysis and homogenization is presented. The system performs sample lysis through a magnetically-actuated beadbeating system followed by centrifugal clarification. Biological validation was performed using Bacillus subtilis spores. The platform is as efficient as in-tube beadbeating lysis and homogenization for nucleic acid extraction, and capable of processing 4 samples to near PCR-ready products in under 6 mins.",
keywords = "Centrifugal, Lysis, Nucleic acid, Sample preparation",
author = "Jonathan Siegrist and Robert Gorkin and Martine Bastien and Gale Stewart and R{\'e}gis Peytavi and Horacio Kido and Michel Bergeron and Marc Madou",
year = "2009",
language = "English",
isbn = "9780979806421",
series = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "1761--1763",
booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",
}