Changes in leaf nitrogen and carbohydrates underlie temperature and CO₂ acclimation of dark respiration in five boreal tree species

We tested the hypothesis that acclimation of foliar dark respiration to CO₂ concentration and temperature is associated with adjustments in leaf structure and chemistry. Populus tremuloides Michx, Betula papyrifera Marsh., Larix laricina (Du Roi) K. Koch, Pinus banksiana Lamb., and Picea mariana (Mill.) B.S.P. were grown from seed in combined CO₂ (370 or 580 μmol mol^-1] and temperature treatments (18/12, 24/18, or 30/24 °C). Temperature and CO₂ effects were predominately independent. Specific respiration rates partially acclimated to warmer thermal environments through downward adjustment in the intercept, but not Q₁₀ of the temperature-response functions. Temperature acclimation of respiration was larger for conifers than broad-leaved species and was associated with pronounced reductions in leaf nitrogen concentrations in conifers at higher growth temperatures. Short-term increases in CO₂ concentration did not inhibit respiration. Growth in the elevated CO₂ concentration reduced leaf nitrogen and increased non-structural carbohydrate concentrations. However, for a given nitrogen concentration, respiration was higher in leaves grown in the elevated CO₂ concentration, as rates increased with increasing carbohydrates. Across species and treatments, respiration rates were a function of both leaf nitrogen and carbohydrate concentrations (R² = 0.71, P < 0.0001). Long-term acclimation of foliar dark respiration to temperature and CO₂ concentration is largely associated with changes in nitrogen and carbohydrate concentrations.