Characterisation of the CD5 cDNA sequence from the tammar wallaby (Macropus eugenii)

L. G. Duncan; J. M. Old

doi:10.1016/j.vetimm.2009.03.005

Characterisation of the CD5 cDNA sequence from the tammar wallaby (Macropus eugenii)

L. G. Duncan, J. M. Old

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Abstract

CD5 has previously been identified in marsupial tissues using anti-human CD5. However, despite the known cross-reactivity of the antibody in marsupial tissues, the cDNA sequence has not previously been characterised in any marsupial. This study has identified the CD5 gene in the opossum genome database and has characterised the CD5 cDNA sequence from the tammar wallaby. Both marsupial CD5 sequences have a high level of sequence identity to known eutherian CD5 sequences, are cysteine-rich and have identical structural motifs to their eutherian homologs. CD5 transcripts were strongly expressed in adult tammar wallaby spleen, mammary node and blood, and expressed at a lower level in liver, kidney and heart tissues. Characterisation of CD5 in marsupials allowed a comparison to the epitope sequence of anti-human CD5 and showed a high level of sequence identity.

Original language	English
Pages (from-to)	117-121
Number of pages	5
Journal	Veterinary Immunology and Immunopathology
Volume	131
Issue number	45323
DOIs	https://doi.org/10.1016/j.vetimm.2009.03.005
Publication status	Published - 2009

Keywords

DNA
analysis
antisense DNA
cacropus eugenii
marsupials
nucleotide sequence
opossums
wallabies

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10.1016/j.vetimm.2009.03.005

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title = "Characterisation of the CD5 cDNA sequence from the tammar wallaby (Macropus eugenii)",

abstract = "CD5 has previously been identified in marsupial tissues using anti-human CD5. However, despite the known cross-reactivity of the antibody in marsupial tissues, the cDNA sequence has not previously been characterised in any marsupial. This study has identified the CD5 gene in the opossum genome database and has characterised the CD5 cDNA sequence from the tammar wallaby. Both marsupial CD5 sequences have a high level of sequence identity to known eutherian CD5 sequences, are cysteine-rich and have identical structural motifs to their eutherian homologs. CD5 transcripts were strongly expressed in adult tammar wallaby spleen, mammary node and blood, and expressed at a lower level in liver, kidney and heart tissues. Characterisation of CD5 in marsupials allowed a comparison to the epitope sequence of anti-human CD5 and showed a high level of sequence identity.",

keywords = "DNA, analysis, antisense DNA, cacropus eugenii, marsupials, nucleotide sequence, opossums, wallabies",

author = "Duncan, {L. G.} and Old, {J. M.}",

year = "2009",

doi = "10.1016/j.vetimm.2009.03.005",

language = "English",

volume = "131",

pages = "117--121",

journal = "Veterinary Immunology and Immunopathology",

issn = "0165-2427",

publisher = "Elsevier",

number = "45323",

}

TY - JOUR

T1 - Characterisation of the CD5 cDNA sequence from the tammar wallaby (Macropus eugenii)

AU - Duncan, L. G.

AU - Old, J. M.

PY - 2009

Y1 - 2009

N2 - CD5 has previously been identified in marsupial tissues using anti-human CD5. However, despite the known cross-reactivity of the antibody in marsupial tissues, the cDNA sequence has not previously been characterised in any marsupial. This study has identified the CD5 gene in the opossum genome database and has characterised the CD5 cDNA sequence from the tammar wallaby. Both marsupial CD5 sequences have a high level of sequence identity to known eutherian CD5 sequences, are cysteine-rich and have identical structural motifs to their eutherian homologs. CD5 transcripts were strongly expressed in adult tammar wallaby spleen, mammary node and blood, and expressed at a lower level in liver, kidney and heart tissues. Characterisation of CD5 in marsupials allowed a comparison to the epitope sequence of anti-human CD5 and showed a high level of sequence identity.

AB - CD5 has previously been identified in marsupial tissues using anti-human CD5. However, despite the known cross-reactivity of the antibody in marsupial tissues, the cDNA sequence has not previously been characterised in any marsupial. This study has identified the CD5 gene in the opossum genome database and has characterised the CD5 cDNA sequence from the tammar wallaby. Both marsupial CD5 sequences have a high level of sequence identity to known eutherian CD5 sequences, are cysteine-rich and have identical structural motifs to their eutherian homologs. CD5 transcripts were strongly expressed in adult tammar wallaby spleen, mammary node and blood, and expressed at a lower level in liver, kidney and heart tissues. Characterisation of CD5 in marsupials allowed a comparison to the epitope sequence of anti-human CD5 and showed a high level of sequence identity.

KW - DNA

KW - analysis

KW - antisense DNA

KW - cacropus eugenii

KW - marsupials

KW - nucleotide sequence

KW - opossums

KW - wallabies

UR - http://handle.uws.edu.au:8081/1959.7/501188

U2 - 10.1016/j.vetimm.2009.03.005

DO - 10.1016/j.vetimm.2009.03.005

M3 - Article

SN - 0165-2427

VL - 131

SP - 117

EP - 121

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 45323

ER -

Characterisation of the CD5 cDNA sequence from the tammar wallaby (Macropus eugenii)

Abstract

Keywords

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