Characteristics of anti-complementary biopolymer extracted from coriolus versicolor

Activation of complementary system (anti-complementary activity) was studied under the influence of biopolymers extracted from fruiting bodies of Coriolus versicolor. The anti-complementary activities of the water-soluble extract (CV-S1) and the ethanol precipitate of the water-soluble extract (CV-S2) were 71.8 and 98.1%, respectively. Activated pathway of complement system was found to be occurred through both the classical and alternative pathways as evidenced by crossed immunoelectrophoresis, where the major pathway was detected to be the classical one. The CV-S2 was fractionated into CV-S2-Fr.I, II and III by gel chromatography. The anti-complementary activities were retained mainly in CV-S2-Fr.I and II. The molecular weights of CV-S2-Fr.I and II were estimated to be about 1200 and 150 kDa, respectively. The structures of CV-S2-Fr.I and II were characterized as 2,3,4,6-tetra-O-methyl, 2,4,6-tri-O-methyl, 2,3,4-tri-O-methyl, 2,3,6-tri-O-methyl, 2,4-di-O-methyl and 2,3-di-O-methyl and 2,3-di-O-methyl-D-glucitol in different proportions.