TY - JOUR
T1 - Chemical composition and cytotoxicity of oils and eremophilanes derived from various parts of Eremophila mitchellii Benth. (Myoporaceae)
AU - Beattie, Karren D.
AU - Waterman, Peter G.
AU - Forster, Paul I.
AU - Thompson, Dion R.
AU - Leach, David N.
PY - 2011
Y1 - 2011
N2 - A detailed investigation of the wood, leaf, branch and root oil of Eremophila mitchellii (Benth.) was carried out by a combination of GC-FID, GC-MS and NMR. The wood oil was composed predominantly of eremophilanes, a rare class of biologically active, bicyclic sesquiterpenoids. The root oil was also found to contain the eremophilanes together with the zizaene sesquiterpene, sesquithuriferone. 9-Hydroxy-1,7(11),9-eremophilatrien-8-one and the known 1(10),11-eremophiladien-9-one (eremophilone), 9-hydroxy-7(11),9-eremophiladien- 8-one (2-hydroxyeremophilone), 8-hydroxy-11-eremophilen-9-one (santalcamphor), 8-hydroxy-10,11-eremophiladien-9-one, sesquithuriferone and 8-hydroxy-1,11- eremophiladien-9-one were purified and elucidated by NMR. Three approaches to the purification of the major eremophilanes from the wood oil are described. (+) Spathulenol, α-pinene, globulol, viridiflorene were the major constituents of the leaf oil. All of the essential oils and the eremophilanes exhibited cytotoxicity against P388D1 mouse lymphoblast cells in-vitro.
AB - A detailed investigation of the wood, leaf, branch and root oil of Eremophila mitchellii (Benth.) was carried out by a combination of GC-FID, GC-MS and NMR. The wood oil was composed predominantly of eremophilanes, a rare class of biologically active, bicyclic sesquiterpenoids. The root oil was also found to contain the eremophilanes together with the zizaene sesquiterpene, sesquithuriferone. 9-Hydroxy-1,7(11),9-eremophilatrien-8-one and the known 1(10),11-eremophiladien-9-one (eremophilone), 9-hydroxy-7(11),9-eremophiladien- 8-one (2-hydroxyeremophilone), 8-hydroxy-11-eremophilen-9-one (santalcamphor), 8-hydroxy-10,11-eremophiladien-9-one, sesquithuriferone and 8-hydroxy-1,11- eremophiladien-9-one were purified and elucidated by NMR. Three approaches to the purification of the major eremophilanes from the wood oil are described. (+) Spathulenol, α-pinene, globulol, viridiflorene were the major constituents of the leaf oil. All of the essential oils and the eremophilanes exhibited cytotoxicity against P388D1 mouse lymphoblast cells in-vitro.
KW - Myoporaceae
KW - cytotoxicity
KW - eremophila (plants)
KW - eremophilanes
KW - essences and essential oils
UR - http://handle.uws.edu.au:8081/1959.7/uws:31244
U2 - 10.1016/j.phytochem.2010.12.011
DO - 10.1016/j.phytochem.2010.12.011
M3 - Article
SN - 0031-9422
VL - 72
SP - 400
EP - 408
JO - Phytochemistry: the international journal of plant chemistry, plant biochemistry and molecular biology
JF - Phytochemistry: the international journal of plant chemistry, plant biochemistry and molecular biology
IS - 45416
ER -