@inproceedings{e104cff58604442e88bc5946ce70d7eb,
title = "Chitosan adhesive films for photochemical tissue bonding",
abstract = "Photochemical tissue bonding (PTB) is a promising sutureless technique for tissue repair. PTB is often achieved by applying a solution of rose bengal (RB) between two tissue edges, which are irradiated by a green laser to crosslink collagen fibers with minimal heat production. In this study, RB has been incorporated in chitosan films to create a novel tissue adhesive that is laser{"}activated. Materials and Methods. Adhesive films, based on chitosan and containing {\^a}ˆ¼0.1wt\% RB were manufactured and bonded to calf intestine by a solid state laser (wavelength={"}‰532{"}‰nm, Fluence{\^a}ˆ¼110{"}‰J/cm 2 , spot size {\^a}ˆ¼5 mm). A single{"}column tensiometer, interfaced with a personal computer, tested the bonding strength. K{"}type thermocouples recorded the temperature (T) at the adhesive{"}tissue interface during laser irradiation. Human fibroblasts were also seeded on the adhesive and cultured for 48 hours to assess cell growth. Results and Conclusion. The RB{"}chitosan adhesive bonded firmly to the intestine(15{\~A}‚{\^A}±2{"}‰kPa,{"}‰n{"}‰={"}‰31). The adhesion strength dropped to0.5{\~A}‚{\^A}±0.1{"}‰kPa (n{"}‰={"}‰8) when the laser was not applied to the adhesive. The average temperature of the adhesive increased from26{"}‰{\~A}‚{\^A}°C to32{"}‰{\~A}‚{\^A}°C during laser exposure. Fibroblasts grew confluent on the adhesive without morphological changes. A new biocompatible chitosan adhesive has been developed that bonds photochemically to tissue with minimal temperature increase.",
author = "Antonio Lauto and Damia Mawad and Matthew Barton and Piller, \{Sabine C.\} and Leonardo Longo",
year = "2011",
doi = "10.1063/1.3626916",
language = "English",
isbn = "9780735409224",
publisher = "American Institute of Physics",
pages = "87--93",
booktitle = "Advances in Laserology: Selected Papers of Laser Florence 2010: The 50th Birthday of Laser Medicine World: Florence, Italy, 5-6 November 2010",
address = "United States",
note = "Laser Florence (Conference) ; Conference date: 05-11-2010",
}