Comparative proteomic analysis of aluminum tolerance in Tibetan wild and cultivated barleys

Huaxin Dai; Fangbin Cao; Xianhong Chen; Mian Zhang; Imrul Mosaddek Ahmed; Zhong-Hua Chen; Chengdao Li; Guoping Zhang; Feibo Wu

doi:10.1371/journal.pone.0063428

Comparative proteomic analysis of aluminum tolerance in Tibetan wild and cultivated barleys

Huaxin Dai
, Fangbin Cao
, Xianhong Chen
, Mian Zhang
, Imrul Mosaddek Ahmed
, Zhong-Hua Chen
, Chengdao Li
, Guoping Zhang
, Feibo Wu

School of Science

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Aluminum (Al) toxicity is a major limiting factor for plant production in acid soils. Wild barley germplasm is rich in genetic diversity and may provide elite genes for crop Al tolerance improvement. The hydroponic-experiments were performed to compare proteomic and transcriptional characteristics of two contrasting Tibetan wild barley genotypes Al- resistant/tolerant XZ16 and Al-sensitive XZ61 as well as Al-resistant cv. Dayton. Results showed that XZ16 had less Al uptake and translocation than XZ61 and Dayton under Al stress. Thirty-five Al-tolerance/resistance-associated proteins were identified and categorized mainly in metabolism, energy, cell growth/division, protein biosynthesis, protein destination/storage, transporter, signal transduction, disease/defense, etc. Among them, 30 were mapped on barley genome, with 16 proteins being exclusively up-regulated by Al stress in XZ16, including 4 proteins (S-adenosylmethionine-synthase 3, ATP synthase beta subunit, triosephosphate isomerase, Bp2A) specifically expressed in XZ16 but not Dayton. The findings highlighted the significance of specific-proteins associated with Al tolerance, and verified Tibetan wild barley as a novel genetic resource for Al tolerance.

Original language	English
Article number	e63428
Pages (from-to)	1-14
Number of pages	14
Journal	PLoS One
Volume	8
Issue number	5
DOIs	https://doi.org/10.1371/journal.pone.0063428
Publication status	Published - 2013

Open Access - Access Right Statement

Copyright: 2013 Dai et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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10.1371/journal.pone.0063428

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title = "Comparative proteomic analysis of aluminum tolerance in Tibetan wild and cultivated barleys",

abstract = "Aluminum (Al) toxicity is a major limiting factor for plant production in acid soils. Wild barley germplasm is rich in genetic diversity and may provide elite genes for crop Al tolerance improvement. The hydroponic-experiments were performed to compare proteomic and transcriptional characteristics of two contrasting Tibetan wild barley genotypes Al- resistant/tolerant XZ16 and Al-sensitive XZ61 as well as Al-resistant cv. Dayton. Results showed that XZ16 had less Al uptake and translocation than XZ61 and Dayton under Al stress. Thirty-five Al-tolerance/resistance-associated proteins were identified and categorized mainly in metabolism, energy, cell growth/division, protein biosynthesis, protein destination/storage, transporter, signal transduction, disease/defense, etc. Among them, 30 were mapped on barley genome, with 16 proteins being exclusively up-regulated by Al stress in XZ16, including 4 proteins (S-adenosylmethionine-synthase 3, ATP synthase beta subunit, triosephosphate isomerase, Bp2A) specifically expressed in XZ16 but not Dayton. The findings highlighted the significance of specific-proteins associated with Al tolerance, and verified Tibetan wild barley as a novel genetic resource for Al tolerance.",

author = "Huaxin Dai and Fangbin Cao and Xianhong Chen and Mian Zhang and Ahmed, \{Imrul Mosaddek\} and Zhong-Hua Chen and Chengdao Li and Guoping Zhang and Feibo Wu",

year = "2013",

doi = "10.1371/journal.pone.0063428",

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T1 - Comparative proteomic analysis of aluminum tolerance in Tibetan wild and cultivated barleys

AU - Dai, Huaxin

AU - Cao, Fangbin

AU - Chen, Xianhong

AU - Zhang, Mian

AU - Ahmed, Imrul Mosaddek

AU - Chen, Zhong-Hua

AU - Li, Chengdao

AU - Zhang, Guoping

AU - Wu, Feibo

PY - 2013

Y1 - 2013

N2 - Aluminum (Al) toxicity is a major limiting factor for plant production in acid soils. Wild barley germplasm is rich in genetic diversity and may provide elite genes for crop Al tolerance improvement. The hydroponic-experiments were performed to compare proteomic and transcriptional characteristics of two contrasting Tibetan wild barley genotypes Al- resistant/tolerant XZ16 and Al-sensitive XZ61 as well as Al-resistant cv. Dayton. Results showed that XZ16 had less Al uptake and translocation than XZ61 and Dayton under Al stress. Thirty-five Al-tolerance/resistance-associated proteins were identified and categorized mainly in metabolism, energy, cell growth/division, protein biosynthesis, protein destination/storage, transporter, signal transduction, disease/defense, etc. Among them, 30 were mapped on barley genome, with 16 proteins being exclusively up-regulated by Al stress in XZ16, including 4 proteins (S-adenosylmethionine-synthase 3, ATP synthase beta subunit, triosephosphate isomerase, Bp2A) specifically expressed in XZ16 but not Dayton. The findings highlighted the significance of specific-proteins associated with Al tolerance, and verified Tibetan wild barley as a novel genetic resource for Al tolerance.

AB - Aluminum (Al) toxicity is a major limiting factor for plant production in acid soils. Wild barley germplasm is rich in genetic diversity and may provide elite genes for crop Al tolerance improvement. The hydroponic-experiments were performed to compare proteomic and transcriptional characteristics of two contrasting Tibetan wild barley genotypes Al- resistant/tolerant XZ16 and Al-sensitive XZ61 as well as Al-resistant cv. Dayton. Results showed that XZ16 had less Al uptake and translocation than XZ61 and Dayton under Al stress. Thirty-five Al-tolerance/resistance-associated proteins were identified and categorized mainly in metabolism, energy, cell growth/division, protein biosynthesis, protein destination/storage, transporter, signal transduction, disease/defense, etc. Among them, 30 were mapped on barley genome, with 16 proteins being exclusively up-regulated by Al stress in XZ16, including 4 proteins (S-adenosylmethionine-synthase 3, ATP synthase beta subunit, triosephosphate isomerase, Bp2A) specifically expressed in XZ16 but not Dayton. The findings highlighted the significance of specific-proteins associated with Al tolerance, and verified Tibetan wild barley as a novel genetic resource for Al tolerance.

UR - http://hdl.handle.net/1959.7/uws:17569

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DO - 10.1371/journal.pone.0063428

M3 - Article

C2 - 23691047

SN - 1932-6203

VL - 8

SP - 1

EP - 14

JO - PLoS One

JF - PLoS One

IS - 5

M1 - e63428

ER -

Comparative proteomic analysis of aluminum tolerance in Tibetan wild and cultivated barleys

Abstract

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