Comparison of mass spectrometry and other techniques for probing interactions between metal complexes and DNA

Thitima Urathamakul; Daniel J. Waller; Jennifer L. Beck; Janice R. Aldrich-Wright; Stephen F. Ralph

Comparison of mass spectrometry and other techniques for probing interactions between metal complexes and DNA

Thitima Urathamakul, Daniel J. Waller, Jennifer L. Beck, Janice R. Aldrich-Wright, Stephen F. Ralph

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24 Citations (Scopus)

Abstract

Electrospray ionization mass spectrometry (ESI-MS) was used to study the binding interactions of two series of ruthenium complexes, [Ru(phen)2L]2+ and [RuLÃ¢â‚¬Â²2(dpqC)]2+, to a double stranded DNA hexadecamer, and derive orders of relative binding affinity. These were shown to be in good agreement with orders of relative binding affinity derived from absorption and circular dichroism (CD) spectroscopic examination of the same systems and from DNA melting curves. However, the extent of luminescence enhancement caused by the addition of DNA to solutions of the ruthenium complexes showed little correlation with orders of binding affinity derived from ESI-MS or any of the other techniques. Overall the results provide support for the validity of using ESI-MS to investigate noncovalent interactions between metal complexes and DNA.

Original language	English
Number of pages	12
Journal	Inorganic Chemistry
Publication status	Published - 2008

Keywords

DNA
DNA-ligand interactions
circular dichroism
electrospray ionization mass spectrometry
ligand binding (biochemistry)
ruthenium compounds

Cite this

@article{d50cb6021d1d4e29a48a8e21033ab831,

title = "Comparison of mass spectrometry and other techniques for probing interactions between metal complexes and DNA",

abstract = "Electrospray ionization mass spectrometry (ESI-MS) was used to study the binding interactions of two series of ruthenium complexes, [Ru(phen)2L]2+ and [RuL{\~A}¢{\^a}‚¬{\^A}²2(dpqC)]2+, to a double stranded DNA hexadecamer, and derive orders of relative binding affinity. These were shown to be in good agreement with orders of relative binding affinity derived from absorption and circular dichroism (CD) spectroscopic examination of the same systems and from DNA melting curves. However, the extent of luminescence enhancement caused by the addition of DNA to solutions of the ruthenium complexes showed little correlation with orders of binding affinity derived from ESI-MS or any of the other techniques. Overall the results provide support for the validity of using ESI-MS to investigate noncovalent interactions between metal complexes and DNA.",

keywords = "DNA, DNA-ligand interactions, circular dichroism, electrospray ionization mass spectrometry, ligand binding (biochemistry), ruthenium compounds",

author = "Thitima Urathamakul and Waller, {Daniel J.} and Beck, {Jennifer L.} and Aldrich-Wright, {Janice R.} and Ralph, {Stephen F.}",

year = "2008",

language = "English",

journal = "Inorganic Chemistry",

issn = "0020-1669",

publisher = "American Chemical Society",

}

TY - JOUR

T1 - Comparison of mass spectrometry and other techniques for probing interactions between metal complexes and DNA

AU - Urathamakul, Thitima

AU - Waller, Daniel J.

AU - Beck, Jennifer L.

AU - Aldrich-Wright, Janice R.

AU - Ralph, Stephen F.

PY - 2008

Y1 - 2008

N2 - Electrospray ionization mass spectrometry (ESI-MS) was used to study the binding interactions of two series of ruthenium complexes, [Ru(phen)2L]2+ and [RuLÃ¢â‚¬Â²2(dpqC)]2+, to a double stranded DNA hexadecamer, and derive orders of relative binding affinity. These were shown to be in good agreement with orders of relative binding affinity derived from absorption and circular dichroism (CD) spectroscopic examination of the same systems and from DNA melting curves. However, the extent of luminescence enhancement caused by the addition of DNA to solutions of the ruthenium complexes showed little correlation with orders of binding affinity derived from ESI-MS or any of the other techniques. Overall the results provide support for the validity of using ESI-MS to investigate noncovalent interactions between metal complexes and DNA.

AB - Electrospray ionization mass spectrometry (ESI-MS) was used to study the binding interactions of two series of ruthenium complexes, [Ru(phen)2L]2+ and [RuLÃ¢â‚¬Â²2(dpqC)]2+, to a double stranded DNA hexadecamer, and derive orders of relative binding affinity. These were shown to be in good agreement with orders of relative binding affinity derived from absorption and circular dichroism (CD) spectroscopic examination of the same systems and from DNA melting curves. However, the extent of luminescence enhancement caused by the addition of DNA to solutions of the ruthenium complexes showed little correlation with orders of binding affinity derived from ESI-MS or any of the other techniques. Overall the results provide support for the validity of using ESI-MS to investigate noncovalent interactions between metal complexes and DNA.

KW - DNA

KW - DNA-ligand interactions

KW - circular dichroism

KW - electrospray ionization mass spectrometry

KW - ligand binding (biochemistry)

KW - ruthenium compounds

UR - http://handle.uws.edu.au:8081/1959.7/487877

M3 - Article

SN - 0020-1669

JO - Inorganic Chemistry

JF - Inorganic Chemistry

ER -

Comparison of mass spectrometry and other techniques for probing interactions between metal complexes and DNA

Abstract

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