Comprehensive coupled reversed-phase reversed-phase separations of a complex isomeric mixture

Michael J. Gray, Alan P. Sweeney, Gary R. Dennis, Patrick J. Slonecker, Ross A. Shalliker

    Research output: Contribution to journalArticle

    Abstract

    Using predictions based on results obtained from Information Theory and Factor Analysis for the two-dimensional separation of a complex isomeric mixture, a practical experimental comprehensive coupled reversed phase-reversed phase chromatographic system was developed. In total four reversed phase-reversed phase systems were studied, each of which theory predicted would be able to resolve essentially equal numbers of components. However, in practice only one of these coupled systems realised the theoretical potential. This system employed as the first dimension, a C18 stationary phase with methanol as the mobile phase and as the second dimension, carbon clad zirconia as the stationary phase and acetonitrile as the mobile phase. In this system, 27 of the 32 isomers of a mixture of oligostyrenes were resolved. Failure of the remaining coupled systems to achieve the theoretical potential was attributed to high solute crowding, low efficiency of separation space utilisation and long analysis times in the second dimension.
    Original languageEnglish
    Pages (from-to)598-604
    Number of pages7
    JournalThe Analyst
    Volume128
    Issue number6
    DOIs
    Publication statusPublished - 2003

    Keywords

    • factor analysis
    • high performance liquid chromatography
    • information theory
    • oligostyrene
    • separation (technology)

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