Cycling of extracellular DNA in the soil environment

David J. Levy-Booth; Rachel G. Campbell; Robert H. Gulden; Miranda M. Hart; Jeff R. Powell; John N. Klironomos; K. Peter Pauls; Clarence J. Swanton; Jack T. Trevors; Kari E. Dunfield

doi:10.1016/j.soilbio.2007.06.020

Cycling of extracellular DNA in the soil environment

David J. Levy-Booth, Rachel G. Campbell, Robert H. Gulden, Miranda M. Hart, Jeff R. Powell, John N. Klironomos, K. Peter Pauls, Clarence J. Swanton, Jack T. Trevors, Kari E. Dunfield

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389 Citations (Scopus)

Abstract

Upon entering the soil environment, extracellular DNA is subjected to dynamic biological, physical, and chemical factors that determine its fate. This review concerns the fate of both recombinant and non-recombinant sources of DNA. A schematic of DNA cycling coupled with genetic transformation is presented to understand its behavior in soil. Extracellular DNA may persist through cation bridging onto soil minerals and humic substances, be enzymatically degraded and restricted by DNases of microbial origin, and/or enter the microbial DNA cycle through natural transformation of competent bacteria. Lateral gene transfer may disseminate DNA through the microbial community. An understanding of DNA cycling is fundamental to elucidating the fate of extracellular DNA in the soil environment.

Original language	English
Number of pages	15
Journal	Soil Biology and Biochemistry
DOIs	https://doi.org/10.1016/j.soilbio.2007.06.020
Publication status	Published - 2007

Keywords

DNA
agriculture
bacteria
degradation
environment
gene transfer

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10.1016/j.soilbio.2007.06.020

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title = "Cycling of extracellular DNA in the soil environment",

abstract = "Upon entering the soil environment, extracellular DNA is subjected to dynamic biological, physical, and chemical factors that determine its fate. This review concerns the fate of both recombinant and non-recombinant sources of DNA. A schematic of DNA cycling coupled with genetic transformation is presented to understand its behavior in soil. Extracellular DNA may persist through cation bridging onto soil minerals and humic substances, be enzymatically degraded and restricted by DNases of microbial origin, and/or enter the microbial DNA cycle through natural transformation of competent bacteria. Lateral gene transfer may disseminate DNA through the microbial community. An understanding of DNA cycling is fundamental to elucidating the fate of extracellular DNA in the soil environment.",

keywords = "DNA, agriculture, bacteria, degradation, environment, gene transfer",

author = "Levy-Booth, {David J.} and Campbell, {Rachel G.} and Gulden, {Robert H.} and Hart, {Miranda M.} and Powell, {Jeff R.} and Klironomos, {John N.} and Pauls, {K. Peter} and Swanton, {Clarence J.} and Trevors, {Jack T.} and Dunfield, {Kari E.}",

year = "2007",

doi = "10.1016/j.soilbio.2007.06.020",

language = "English",

journal = "Soil Biology and Biochemistry",

issn = "0038-0717",

publisher = "Elsevier",

}

TY - JOUR

T1 - Cycling of extracellular DNA in the soil environment

AU - Levy-Booth, David J.

AU - Campbell, Rachel G.

AU - Gulden, Robert H.

AU - Hart, Miranda M.

AU - Powell, Jeff R.

AU - Klironomos, John N.

AU - Pauls, K. Peter

AU - Swanton, Clarence J.

AU - Trevors, Jack T.

AU - Dunfield, Kari E.

PY - 2007

Y1 - 2007

N2 - Upon entering the soil environment, extracellular DNA is subjected to dynamic biological, physical, and chemical factors that determine its fate. This review concerns the fate of both recombinant and non-recombinant sources of DNA. A schematic of DNA cycling coupled with genetic transformation is presented to understand its behavior in soil. Extracellular DNA may persist through cation bridging onto soil minerals and humic substances, be enzymatically degraded and restricted by DNases of microbial origin, and/or enter the microbial DNA cycle through natural transformation of competent bacteria. Lateral gene transfer may disseminate DNA through the microbial community. An understanding of DNA cycling is fundamental to elucidating the fate of extracellular DNA in the soil environment.

AB - Upon entering the soil environment, extracellular DNA is subjected to dynamic biological, physical, and chemical factors that determine its fate. This review concerns the fate of both recombinant and non-recombinant sources of DNA. A schematic of DNA cycling coupled with genetic transformation is presented to understand its behavior in soil. Extracellular DNA may persist through cation bridging onto soil minerals and humic substances, be enzymatically degraded and restricted by DNases of microbial origin, and/or enter the microbial DNA cycle through natural transformation of competent bacteria. Lateral gene transfer may disseminate DNA through the microbial community. An understanding of DNA cycling is fundamental to elucidating the fate of extracellular DNA in the soil environment.

KW - DNA

KW - agriculture

KW - bacteria

KW - degradation

KW - environment

KW - gene transfer

UR - http://handle.uws.edu.au:8081/1959.7/511827

U2 - 10.1016/j.soilbio.2007.06.020

DO - 10.1016/j.soilbio.2007.06.020

M3 - Article

SN - 0038-0717

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

ER -

Cycling of extracellular DNA in the soil environment

Abstract

Keywords

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