TY - JOUR
T1 - Design and fabrication of a COP-based microfluidic chip : chronoamperometric detection of Troponin T
AU - Abad, Llibertat
AU - Javier del Campo, Francisco
AU - Xavier Munoz, Francesc
AU - Fernandez, Luis J.
AU - Calavia, Daniel
AU - Colom, Gloria
AU - Salvador, Juan P.
AU - Pilar Marco, Maria
AU - Escamilla-Gomez, Vanessa
AU - Esteban-Fernandez de Avila, Berta
AU - Campuzano, Susana
AU - Pedrero, Maria
AU - Pingarron, Jose M.
AU - Godino, Neus
AU - Gorkin, Robert
AU - Ducree, Jens
PY - 2012
Y1 - 2012
N2 - This work demonstrates the design and fabrication of an all cyclo-olefin polymer based microfluidic device capable of capturing magnetic beads and performing electrochemical detection in a series of gold electrodes. The size of chip is of a microscope slide and features six independent measuring cells for multianalyte detection purposes. The aim of this work is to show that rapid prototyping techniques can be instrumental in the development of novel bioassays, particularly in clinical diagnosis applications. We show the successful determination of troponin-T, a cardiac disease marker, in the clinically relevant range of 0.05-1.0 ng/mL. This methodology achieves a detection limit of 0.017 ng/mL in PBS solutions, and is capable of detecting less than 1 ng/mL in a 1:50 human serum dilution.
AB - This work demonstrates the design and fabrication of an all cyclo-olefin polymer based microfluidic device capable of capturing magnetic beads and performing electrochemical detection in a series of gold electrodes. The size of chip is of a microscope slide and features six independent measuring cells for multianalyte detection purposes. The aim of this work is to show that rapid prototyping techniques can be instrumental in the development of novel bioassays, particularly in clinical diagnosis applications. We show the successful determination of troponin-T, a cardiac disease marker, in the clinically relevant range of 0.05-1.0 ng/mL. This methodology achieves a detection limit of 0.017 ng/mL in PBS solutions, and is capable of detecting less than 1 ng/mL in a 1:50 human serum dilution.
UR - https://hdl.handle.net/1959.7/uws:72599
U2 - 10.1002/elps.201200225
DO - 10.1002/elps.201200225
M3 - Article
SN - 0173-0835
VL - 33
SP - 3187
EP - 3194
JO - Electrophoresis
JF - Electrophoresis
IS - 21
ER -
