Detection of Panax quinquefolius in Panax ginseng using 'subtracted diversity array'

Linhai Niu, Nitin Mantri, Chun Guang Li, Charlie Xue, Hans Wohlmuth, Edwin C. K. Pang

    Research output: Contribution to journalArticlepeer-review

    19 Citations (Scopus)

    Abstract

    BACKGROUND: Food adulteration remains a major global concern. DNA fingerprinting has several advantages over chemical and morphological identification techniques. DNA microarray-based fingerprinting techniques have not been used previously to detect adulteration involving dried commercial samples of closely related species. Here we report amplification of low-level DNA obtained from dried commercial ginseng samples using the Qiagen ™ REPLI-g ® Kit. Further, we used a subtracted diversity array (SDA) to fingerprint the two ginseng species, Panax ginseng and Panax quinquefolius, that are frequently mixed for adulteration. RESULTS: The two ginseng species were successfully discriminated using SDA. Further, SDA was sensitive enough to detect a deliberate adulteration of 10% P. quinquefolius in P. ginseng. Thirty-nine species-specific features including 30 P. ginseng-specific and nine P. quinquefolius-specific were obtained. This resulted in a feature polymorphism rate of 10.5% from the 376 features used for fingerprinting the two ginseng species. The functional characterization of 14 Panax species-specific features by sequencing revealed one putative ATP synthase, six putative uncharacterized proteins, and two retroelements to be different in these two species. CONCLUSION: SDA can be employed to detect adulterations in a broad range of plant samples.
    Original languageEnglish
    Pages (from-to)1310-1315
    Number of pages6
    JournalJournal of the Science of Food and Agriculture
    Volume91
    Issue number7
    DOIs
    Publication statusPublished - 2011

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