Development of DNA-immobilised chromatographic stationary phases for optical resolution and DNA-affinity comparison of metal complexes

Janice R. Aldrich-Wright, Ivan Greguric, Robert S. Vagg, Kymberley Vickery, Peter A. Williams

    Research output: Contribution to journalArticlepeer-review

    41 Citations (Scopus)

    Abstract

    Two chiral stationary phases which exploit DNA as a chiral discriminator have been developed. A covalentlybound DNA stationary phase for HPLC applications was used to optically resolve [Ru(dipyrido[6,7-d:2',3'-f]-qulnoxaline)3]2+ and [Ru(1,10-phenanthroline)3]2+ complex ions. This shows that the column retention times are influenced both by pH and by the size of the aromatic ligands. DNA-immobilised on cellulose paper proved effective for simultaneously comparing the relative retention of a number of metal complexes and R F data correlate well with the degree of aromatic area in the complexes available for intercalation into DNA.
    Original languageEnglish
    Pages (from-to)436-443
    Number of pages8
    JournalJournal of Chromatography A
    Volume718
    Issue number2
    DOIs
    Publication statusPublished - 1995

    Keywords

    • DNA, affinity comparison

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