Distribution and persistence of Amanita muscaria genotypes in Australian Pinus radiata plantations

Nicole A. Sawyer, Susan M. Chambers, John W. G. Cairney

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Basidiomes of Amanita muscaria were collected from sites in three 30–36-yr-old Pinus radiata plantations in New South Wales, Australia. Following DNA extraction from basidiome stipes, inter-simple sequence repeat PCR using the degenerate primers 5[prime prime or minute]DHB(CGA)5 and 5[prime prime or minute]DDB(CCA)5, was used to identify the mycelial genotypes from which basidiomes arose. Each site was found to contain 8–10 A. muscaria genotypes, with six genotypes common to all three sites and one common to two sites. The presence of common genotypes at the three sites is taken to indicate that they were introduced as vegetative inocula when seedlings were planted and have persisted for < 36 yr.
    Original languageEnglish
    Number of pages5
    JournalMycological Research
    Publication statusPublished - 2001

    Keywords

    • Amanita muscaria
    • Australia
    • Genetics
    • Pinus radiata
    • basidiomes
    • genotypes

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